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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-ERK1 + ERK2 抗体 [EPR17526] (ab184699)

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Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Flow Cytometry (Intracellular) - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Immunoprecipitation - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17526] to ERK1 + ERK2
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-ERK1 + ERK2 antibody [EPR17526]
    ERK1 + ERK2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17526] to ERK1 + ERK2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IP, ICC/IF, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human ERK1 full length recombinant protein; Human ERK2 full length recombinant protein; A431, Jurkat, HeLa, HepG2, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, heart, kidney and spleen lysates. ICC/IF: HeLa cells. Flow Cyt (intra): A431 cells. IP. PC-12 whole cell extract.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184699の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/440.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/70.
ICC/IF (1)
1/250.
WB
1/10000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 43, 41 kDa).
特記事項
Flow Cyt (Intra)
1/440.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/70.
ICC/IF
1/250.
WB
1/10000. Detects a band of approximately 44, 42 kDa (predicted molecular weight: 43, 41 kDa).

ターゲット情報

  • 機能

    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

  • 配列類似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.

  • ドメイン

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

  • 翻訳後修飾

    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 製品の状態

    Mainly expressed in the cytoplasm and only localizes to the nucleus with treatment.

  • 別名

    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-1 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase 3 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • p44ERK1 antibody
    • p44MAPK antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

画像

  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Mouse kidney lysates
    Lane 4 : Mouse spleen lysates
    Lane 5 : Rat brain lysates
    Lane 6 : Rat heart lysates
    Lane 7 : Rat kidney lysates
    Lane 8 : Rat spleen lysates
    Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43, 41 kDa
    Observed band size: 44, 42 kDa why is the actual band size different from the predicted?



    44kDa band represents ERK1. 42kDa band represents ERK2.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MAPK1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 43, 41 kDa
    Observed band size: 44 kDa why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab184699 observed at 44 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab184699 Anti-ERK1 + ERK2 antibody [EPR17526] was shown to specifically react with ERK2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265052 (knockout cell lysate ab257525) was used. Wild-type and ERK2 knockout samples were subjected to SDS-PAGE. ab184699 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)This image is courtesy of an Abreview submitted by Kirk Mcmanus.

    Ab184699 staining ERK1 + ERK2 in HeLa cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution + Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43, 41 kDa
    Observed band size: 44 kDa why is the actual band size different from the predicted?



    Recombinant full length ERK1 protein (ab43623) contains aa1-379.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ERK1 + ERK2 with ab184699 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab184699 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution + Recombinant Human ERK2 protein (ab43625) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43, 41 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Recombinant full length ERK2 protein (ab43625) contains aa1-360.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/50000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysates
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysates
    Lane 5 : Human fetal brain lysates
    Lane 6 : Human fetal heart lysates
    Lane 7 : Human fetal kidney lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43, 41 kDa
    Observed band size: 42,44 kDa why is the actual band size different from the predicted?



    44kDa band represents ERK1. 42kDa band represents ERK2.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Western blot - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    All lanes : Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699) at 1/10000 dilution

    Lane 1 : Human fetal brain lysates
    Lane 2 : Human fetal heart lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 43, 41 kDa
    Observed band size: 42,44 kDa why is the actual band size different from the predicted?



    44kDa band represents ERK1. 42kDa band represents ERK2.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry (Intracellular) - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Flow Cytometry (Intracellular) - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

    Intracellular flow cytometric analysis of A431 (Human epidermoid carcinoma) cells labeling ERK1 + ERK2 with ab184699 at 1/440 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Immunoprecipitation - Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

    ERK1 + ERK2 were immunoprecipitated from 1mg of PC-12 (Rat adrenal gland pheochromocytoma) whole cell extract with ab184699 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab184699 at 1/5000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: PC-12 whole cell extract. Lane 2: PBS instead of PC-12 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    44kDa band represents ERK1. 42kDa band represents ERK2.

  • Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
    Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)

参考文献 (259)

ab184699 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184699 は 259 報の論文で使用されています。

  • Abdelaziz M  et al. Agomelatine improves memory and learning impairments in a rat model of LPS-induced neurotoxicity by modulating the ERK/SorLA/BDNF/TrkB pathway. Naunyn Schmiedebergs Arch Pharmacol 397:1701-1714 (2024). PubMed: 37712973
  • Li M  et al. Serabelisib regulates GSDMD-mediated pyroptosis, apoptosis and migration of hepatoma cells via the PI3K/Akt/E-cadherin signaling pathway. Adv Clin Exp Med 33:171-181 (2024). PubMed: 37486695
  • Chong PSY  et al. Epigenetic dysregulation of eukaryotic initiation factor 3 subunit E (eIF3E) by lysine methyltransferase REIIBP confers a pro-inflammatory phenotype in t(4;14) myeloma. Haematologica 109:1893-1908 (2024). PubMed: 38124661
  • Mu M  et al. 3-Bromopyruvate overcomes cetuximab resistance in human colorectal cancer cells by inducing autophagy-dependent ferroptosis. Cancer Gene Ther 30:1414-1425 (2023). PubMed: 37558749
  • Yuan ZL  et al. Inhibition of ERK downregulates autophagy via mitigating mitochondrial fragmentation to protect SH-SY5Y cells from OGD/R injury. Cell Commun Signal 21:204 (2023). PubMed: 37580749
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Immunocytochemistry/ Immunofluorescence abreview for Anti-ERK1 + ERK2 antibody [EPR17526]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

投稿 Jul 26 2017

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