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リコンビナントRabMAb

Anti-ERG 抗体 [EPR3863] (ab110639)

Reviews (2)Q&A (1)References (31)
Western blot - Anti-ERG antibody [EPR3863] (ab110639)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (ab110639)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (ab110639)
  • Anti-ERG antibody [EPR3863] (ab110639)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3863] to ERG
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-ERG antibody [EPR3863]
    ERG 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3863] to ERG

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat, HT29, and MCF7 cell lysates. IHC-P: Human spleen and tonsil tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab110639の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.

  • 関連疾患

    Defects in ERG are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1.
    Note=Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4.

  • 配列類似性

    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.

  • 細胞内局在

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Avian erythroblastosis virus E-26 (v-ets) oncogene related antibody
      • D030036I24Rik antibody
      • Erg 3 antibody
      • Erg antibody
      • ERG/EWS fusion gene, included antibody
      • ERG/FUS fusion gene, included antibody
      • ERG/TMPSSR2 fusion gene, included antibody
      • ERG_HUMAN antibody
      • ERG1, included antibody
      • ERG2, included antibody
      • ets related antibody
      • ETS-related gene antibody
      • KCNH2 antibody
      • Oncogene ERG antibody
      • p55 antibody
      • TMPRSS2/ERG fusion antibody
      • transcriptional regulator ERG (transforming protein ERG) antibody
      • Transcriptional regulator ERG antibody
      • Transforming protein ERG antibody
      • v ets avian erythroblastosis virus E26 oncogene antibody
      • v ets avian erythroblastosis virus E26 oncogene related antibody
      • v ets erythroblastosis virus E26 oncogene homolog antibody
      • v ets erythroblastosis virus E26 oncogene like antibody
      • v ets erythroblastosis virus E26 oncogene like isoform 2 antibody
      • v-ets erythroblastosis virus E26 oncogene antibody
      • v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody
      • V-ets erythroblastosis virus E26 oncogene like (Avian), isoform CRA_e antibody
      see all

    画像

    • Western blot - Anti-ERG antibody [EPR3863] (ab110639)
      Western blot - Anti-ERG antibody [EPR3863] (ab110639)
      All lanes : Anti-ERG antibody [EPR3863] (ab110639) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HT29 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 55 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (ab110639)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (ab110639)

      ab110639 at 1/100 dilution staining ERG in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (ab110639)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3863] (ab110639)

      ab110639 at 1/100 dilution staining ERG in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-ERG antibody [EPR3863] (ab110639)
      Anti-ERG antibody [EPR3863] (ab110639)

    参考文献 (31)

    ab110639 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab110639 は 31 報の論文で使用されています。

    • Ben Dhaou C  et al. Chemerin regulates normal angiogenesis and hypoxia-driven neovascularization. Angiogenesis 25:159-179 (2022). PubMed: 34524600
    • Ben Dhaou C  et al. CCRL2 Modulates Physiological and Pathological Angiogenesis During Retinal Development. Front Cell Dev Biol 9:808455 (2021). PubMed: 35004698
    • Ren AA  et al. PIK3CA and CCM mutations fuel cavernomas through a cancer-like mechanism. Nature 594:271-276 (2021). PubMed: 33910229
    • Zink J  et al. EVL regulates VEGF receptor-2 internalization and signaling in developmental angiogenesis. EMBO Rep 22:e48961 (2021). PubMed: 33512764
    • Han D  et al. Susceptibility-Associated Genetic Variation in NEDD9 Contributes to Prostate Cancer Initiation and Progression. Cancer Res 81:3766-3776 (2021). PubMed: 33632899
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    CUT&Tag abreview for Anti-ERG antibody [EPR3863]

     Excellent
    Abreviews
    abreview image
    Application
    CUT&Tag
    Sample
    Human Cell lysate - nuclear (Human Aortic Endothelial Cells)
    Specification
    Human Aortic Endothelial Cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 03 2024

    IHC - Wholemount abreview for Anti-ERG antibody [EPR3863] - ChIP Grade

     Good
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E10.5)
    Specification
    E10.5
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Sophie Astrof

    Verified customer

    投稿 Jan 31 2017

    Question

    I'm worling on multiple myeloma and for or IHC we are now using a CD31 marker to look for angionesis in bone marrow. To improve our staining i would like to use the ERG antibody. Is it possible to get a sample for trying? We are working on mice, so i think the ab110639 woud be good for are test.

    Read More
    Answer

    Thank you very much for your interest in ab110639.

    To our knowledge,ab110639 has not been tested in Mouse. Therefore, I can offer a discount off a future purchase if you buy ab110639 now, test it inMouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 freeprimary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab110639 in Mouse. I will then send a discount code. This code must be issued before purchasing ab110639 so please wait for my reply before ordering.

    2. Purchase ab110639 either by phone, fax, or online (www.abcam.com).

    3. Test it in Mouse.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab110639 turns out to be unsuitable for Mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com