Anti-eRF1 抗体 (ab31799)
Key features and details
- Rabbit polyclonal to eRF1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-eRF1 antibody
eRF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eRF1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit, Cow, Xenopus laevis, Zebrafish
-
免疫原
Synthetic peptide corresponding to Human eRF1 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab31798) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31799の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
|
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 49 kDa). |
|
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
-
機能
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. -
配列類似性
Belongs to the eukaryotic release factor 1 family. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 541077 Cow
- Entrez Gene: 2107 Human
- Entrez Gene: 225363 Mouse
- Entrez Gene: 100009553 Rabbit
- Entrez Gene: 307503 Rat
- Entrez Gene: 399462 Xenopus laevis
- Omim: 600285 Human
- SwissProt: P62495 Human
see all -
別名
- Cl1 protein antibody
- D5S1995 antibody
- ERF antibody
see all
画像
-
Western blot - Anti-eRF1 antibody (ab31799)All lanes : Anti-eRF1 antibody (ab31799) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Western blot - Anti-eRF1 antibody (ab31799)All lanes : Anti-eRF1 antibody (ab31799) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 7 : Liver (Rat) Tissue Lysate
Lane 8 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (ab31799)ICC/IF image of ab31799 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31799, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab31799 は 3 報の論文で使用されています。
- Huang L et al. Targeting Translation Termination Machinery with Antisense Oligonucleotides for Diseases Caused by Nonsense Mutations. Nucleic Acid Ther 29:175-186 (2019). PubMed: 31070517
- Wang X et al. Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor of Programmed -1 Ribosomal Frameshifting. Cell 176:625-635.e14 (2019). PubMed: 30682371
- Liu P et al. Deficiency in a glutamine-specific methyltransferase for release factor causes mouse embryonic lethality. Mol Cell Biol 30:4245-53 (2010). WB, IP ; Human . PubMed: 20606008