Anti-eRF1 抗体 (ab153731)
Key features and details
- Rabbit polyclonal to eRF1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-eRF1 antibody
eRF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eRF1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Xenopus tropicalis
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免疫原
Synthetic peptide within Human eRF1 aa 388-437 (C terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- 293T; A431; H1299; HeLa; HepG2; MOLT4 and Raji cell lysates; SW480 xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153731の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. -
配列類似性
Belongs to the eukaryotic release factor 1 family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 541077 Cow
- Entrez Gene: 2107 Human
- Entrez Gene: 225363 Mouse
- Entrez Gene: 100009553 Rabbit
- Entrez Gene: 307503 Rat
- Omim: 600285 Human
- SwissProt: P62495 Human
- SwissProt: Q8BWY3 Mouse
see all -
別名
- Cl1 protein antibody
- D5S1995 antibody
- ERF antibody
see all
画像
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Western blot - Anti-eRF1 antibody (ab153731)Anti-eRF1 antibody (ab153731) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eRF1 antibody (ab153731)Immunohistochemical analysis of paraffin embedded SW480 xenograft labeling eRF1 using ab153731 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-eRF1 antibody (ab153731)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labeling eRF1 using ab153731 at 1/200 dilution.
The image in the right panel was merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab153731 は論文での使用が確認できていません。