Anti-ERF 抗体 (ab153726)
Key features and details
- Rabbit polyclonal to ERF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ERF antibody
ERF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ERF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
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免疫原
Recombinant fragment corresponding to Human ERF aa 1-265.
Database link: P50548 -
ポジティブ･コントロール
- WB: A431 cell lysate IHC-P: U373 xenograft, mouse testis. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153726の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/10000. Predicted molecular weight: 59 kDa.
|
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| IHC-P |
1/100 - 1/1000.
|
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| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/10000. Predicted molecular weight: 59 kDa. |
|
IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Potent transcriptional repressor that binds to the H1 element of the Ets2 promoter. May regulate other genes involved in cellular proliferation. Required for extraembryonic ectoderm differentiation, ectoplacental cone cavity closure, and chorioallantoic attachment (By similarity). May be important for regulating trophoblast stem cell differentiation. -
組織特異性
Highest levels in testis, ovary, pancreas, and heart. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
翻訳後修飾
Phosphorylated by multiple kinases including MAPK1/ERK2 at THR-526. Phosphorylation regulates the activity of ERF. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 789798 Cow
- Entrez Gene: 2077 Human
- Entrez Gene: 13875 Mouse
- Entrez Gene: 292721 Rat
- Omim: 611888 Human
- SwissProt: P50548 Human
- SwissProt: P70459 Mouse
- Unigene: 655969 Human
see all -
別名
- ERF antibody
- ERF_HUMAN antibody
- ETS domain containing transcription factor ERF antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERF antibody (ab153726)
Paraffin embedded mouse testis tissue stained for ERF using ab153726 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-ERF antibody (ab153726)HeLa cells stained for ERF (green) using ab153726 at 1/500 dilution in ICC/IF.
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Western blot - Anti-ERF antibody (ab153726)Anti-ERF antibody (ab153726) at 1/1000 dilution + A431 cell lysate at 30 µg
Predicted band size: 59 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERF antibody (ab153726)Immunohistochemical analysis of paraffin-embedded U373 xenograft labeling ERF using ab153726 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab153726 は 1 報の論文で使用されています。
- Zadora PK et al. Integrated Phosphoproteome and Transcriptome Analysis Reveals Chlamydia-Induced Epithelial-to-Mesenchymal Transition in Host Cells. Cell Rep 26:1286-1302.e8 (2019). PubMed: 30699355