AB137033

Anti-ERCC8 抗体 [EPR9237]

Anti-ERCC8 antibody [EPR9237]

20ul selling size
RabMAb
Recombinant
KO Validated
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(16 Publications)

Rabbit Recombinant Monoclonal ERCC8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 16 publications.

別名を表示する

CKN1, CSA, ERCC8, DNA excision repair protein ERCC-8, Cockayne syndrome WD repeat protein CSA

3 Images

Lab

Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] (AB137033)

Purified ab137033 at 1/80 dilution (2μg) immunoprecipitating ERCC8 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab137033 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137033 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 44 kDa

All lanes:

Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] (ab137033)

Predicted band size: 44 kDa

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Western blot - Anti-ERCC8 antibody [EPR9237] (AB137033)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : ERCC8 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : Molt-4 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab137033 observed at 44 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab137033 was shown to recognize ERCC8 when ERCC8 knockout samples were used, along with additional cross-reactive bands. Wild-type and ERCC8 knockout samples were subjected to SDS-PAGE. ab137033 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively, and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ERCC8 antibody [EPR9237] (ab137033)

Predicted band size: 44 kDa

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Unknown

Western blot - Anti-ERCC8 antibody [EPR9237] (AB137033)

All lanes:

Western blot - Anti-ERCC8 antibody [EPR9237] (ab137033) at 1/1000 dilution

Lane 1:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 3:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Predicted band size: 44 kDa

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR9237

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ERCC8 also known as CSA protein plays an essential role in the repair of transcription-coupled nucleotide excision repair (TC-NER) pathways. This protein has a mass of about 44 kDa. Expression of ERCC8 is found in several tissues notably in the skin liver and nervous system. It helps in recognizing and repairing DNA damage that occurs during transcription.

Biological function summary

ERCC8 forms a part of the CSA complex which includes other proteins involved in the TC-NER pathway. The CSA complex targets the repair machinery to the site of transcription-blocking lesions. Identifying these lesions is critical to resuming normal transcription processes. The CSA protein therefore actively contributes to maintaining genomic stability by facilitating the correction of transcription-coupled DNA damage.

Pathways

ERCC8 participates directly in the nucleotide excision repair (NER) pathway specifically in its transcription-coupled repair subprocess. This pathway is integral to correcting helix-distorting DNA lesions that impede transcription. ERCC8 interacts closely with the Cockayne syndrome B (CSB) protein and RNA polymerase II during the repair process. This collaboration is important in modulating the restart of transcription post-repair.

Mutations in ERCC8 lead to Cockayne syndrome (CS) characterized by growth defects neurological dysfunction and photosensitivity. ERCC8 alterations are linked primarily to Cockayne syndrome group A. The protein is also involved in repair mechanisms tied to UV-sensitive syndrome. In both conditions faulty ERCC8 function can disrupt normal DNA repair processes leading to severe clinical manifestations associated with DNA repair failure. The role of CSA alongside CSB mutations further highlights its importance in disease phenotypes and the need for effective DNA repair.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes (PubMed : 12732143, PubMed : 16751180, PubMed : 16964240, PubMed : 32142649, PubMed : 34526721, PubMed : 38316879, PubMed : 38600235, PubMed : 38600236). Following recruitment to lesion-stalled RNA polymerase II (Pol II), the CSA complex mediates ubiquitination of Pol II subunit POLR2A/RPB1 at 'Lys-1268', a critical TC-NER checkpoint, governing RNA Pol II stability and initiating DNA damage excision by TFIIH recruitment (PubMed : 12732143, PubMed : 16751180, PubMed : 16964240, PubMed : 32142649, PubMed : 32355176, PubMed : 34526721, PubMed : 38316879, PubMed : 38600235, PubMed : 38600236). The CSA complex also promotes the ubiquitination and subsequent proteasomal degradation of ERCC6/CSB in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair (PubMed : 16751180). Also plays a role in DNA double-strand breaks (DSSBs) repair by non-homologous end joining (NHEJ) (PubMed : 29545921).

See full target information ERCC8

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation : PubMed40374849

2025

Cockayne syndrome mice reflect human kidney disease and are defective in de novo NAD biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Komal Pekhale,Vinod Tiwari,Mansoor Hussain,Christy C Bridges,Deborah L Croteau,Moshe Levi,Avi Z Rosenberg,Briana Santo,Xiaoping Yang,Tomasz Kulikowicz,Xiaoxin X Wang,Jong-Hyuk Lee,Vilhelm A Bohr

Nucleic acids research 53: PubMed40219966

2025

Poly-(ADP-ribose) serves as a scaffold for the methyltransferase METTL3/14 complex in the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Gonzalez-Leal,Jin Cai,Bram A F J de Groot,Andreas Wegerer,Julia Preisser,Martijn S Luijsterburg,Andreas G Ladurner

Frontiers in oncology 14:1476100 PubMed39582530

2024

Characterization of the function and clinical value of ERCC family genes in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Lu,Guoxin Hou

Nature communications 15:7089 PubMed39154022

2024

Coordination of transcription-coupled repair and repair-independent release of lesion-stalled RNA polymerase II.

Applications

Unspecified application

Species

Unspecified reactive species

Yongchang Zhu,Xiping Zhang,Meng Gao,Yanchao Huang,Yuanqing Tan,Avital Parnas,Sizhong Wu,Delin Zhan,Sheera Adar,Jinchuan Hu

Cell reports methods 4:100674 PubMed38176411

2024

Live-cell imaging of endogenous CSB-mScarletI as a sensitive marker for DNA-damage-induced transcription stress.

Applications

Unspecified application

Species

Unspecified reactive species

Di Zhou,Qing Yu,Roel C Janssens,Jurgen A Marteijn

Nucleic acids research 51:6238-6245 PubMed37144462

2023

Nucleotide excision repair in Human cell lines lacking both XPC and CSB proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A Lindsey-Boltz,Yanyan Yang,Cansu Kose,Nazli Deger,Khagani Eynullazada,Hiroaki Kawara,Aziz Sancar

Cells 11: PubMed36231052

2022

A Novel Missense Mutation in Co-Segregates with Cerebellar Ataxia in a Consanguineous Pakistani Family.

Applications

Unspecified application

Species

Unspecified reactive species

Zeeshan Gauhar,Leon Tejwani,Uzma Abdullah,Sadia Saeed,Shagufta Shafique,Mazhar Badshah,Jungmin Choi,Weilai Dong,Carol Nelson-Williams,Richard P Lifton,Janghoo Lim,Ghazala K Raja

Nature communications 13:974 PubMed35190564

2022

Global and transcription-coupled repair of 8-oxoG is initiated by nucleotide excision repair proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Namrata Kumar,Arjan F Theil,Vera Roginskaya,Yasmin Ali,Michael Calderon,Simon C Watkins,Ryan P Barnes,Patricia L Opresko,Alex Pines,Hannes Lans,Wim Vermeulen,Bennett Van Houten

Cell 180:1228-1244.e24 PubMed32142649

2020

Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Nakazawa,Yuichiro Hara,Yasuyoshi Oka,Okiru Komine,Diana van den Heuvel,Chaowan Guo,Yasukazu Daigaku,Mayu Isono,Yuxi He,Mayuko Shimada,Kana Kato,Nan Jia,Satoru Hashimoto,Yuko Kotani,Yuka Miyoshi,Miyako Tanaka,Akira Sobue,Norisato Mitsutake,Takayoshi Suganami,Akio Masuda,Kinji Ohno,Shinichiro Nakada,Tomoji Mashimo,Koji Yamanaka,Martijn S Luijsterburg,Tomoo Ogi

Nucleic acids research 48:2473-2485 PubMed31970402

2020

Cockayne syndrome group A and B proteins function in rRNA transcription through nucleolin regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Mustafa N Okur,Jong-Hyuk Lee,Wasif Osmani,Risako Kimura,Tyler G Demarest,Deborah L Croteau,Vilhelm A Bohr

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERCC8 抗体 [EPR9237]

Anti-ERCC8 antibody [EPR9237]

Immunoprecipitation - Anti-ERCC8 antibody [EPR9237] (AB137033)

Western blot - Anti-ERCC8 antibody [EPR9237] (AB137033)

Western blot - Anti-ERCC8 antibody [EPR9237] (AB137033)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (16)

Abcam product promise