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リコンビナントRabMAb

Anti-ErbB4 / HER4 (phospho Y1162) 抗体 [EP2270Y] (ab68478)

Reviews (1) Submit a question References (3)
Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
  • Immunoprecipitation - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
  • Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2270Y] to ErbB4 / HER4 (phospho Y1162)
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y]
    ErbB4 / HER4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2270Y] to ErbB4 / HER4 (phospho Y1162)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: A431 treated with 100 ng/ml EGF. IHC-P: Human breast carcinoma tissue. IP: A431 cells.

  • 特記事項

    This product has switched from a hybridoma to recombinant production method on 31st May 2023.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68478の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/5000. Predicted molecular weight: 200 kDa.
IP
1/20.
IHC-P (1)
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/5000. Predicted molecular weight: 200 kDa.
IP
1/20.
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Specifically binds and is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. The C-terminal fragment (CTF) of isoform JMA-A CYT-2 (containing E4ICD2) can stimulate transcription in the presence of YAP1. ERBB4 intracellular domain is involved in the regulation of cell growth. Conflicting reports are likely due at least in part to the opposing effects of the isoform-specific and nuclear-translocated ERBB4 intracellular domains (E4ICD1 and E4ICD2). Overexpression studies in epithelium show growth inhibition using E4ICD1 and increased proliferation using E4ICD2. E4ICD2 has greater in vitro kinase activity than E4ICD1. The kinase activity is required for the nuclear translocation of E4ICD2.

  • 組織特異性

    Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Isoform JM-A CYT-1 and isoform JM-A CYT-2 but not isoform JM-B CYT-1 and isoform JM-B CYT-2 are processed by ADAM17. Proteolytic processing in response to ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation results in the production of 120 kDa soluble receptor forms and intermediate membrane-anchored 80 kDa fragments (m80HER4), which are further processed by a presenilin-dependent gamma-secretase to release the respective cytoplasmic intracellular domain E4ICD (either E4ICD1/s80Cyt1 or E4ICD2/s80Cyt2). Membrane-anchored 80 kDa fragments of the processed isoform JM-A CYT-1 are more readily degraded by the proteasome than fragments of isoform JM-A CYT-2 suggesting a prevalence of E4ICD2 over E4ICD1.
    Ligand-binding increases phosphorylation on tyrosine residues. Isoform JM-A CYT-2 is constitutively phosphorylated on tyrosine residues in a ligand-independent manner. E4ICD2 but not E4ICD1 is phosphorylated on tyrosine residues.
    Ubiquitinated. The ERBB4 intracellular domain is ubiquitinated and targeted to proteosomal degradation during mitosis mediated by the APC/C complex. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are ubiquitinated by WWP1. The ERBB4 intracellular domain (E4ICD1) is ubiquitinated, and this involves NEDD4.

  • 細胞内局在

    Membrane and Nucleus. Following proteolytical processing E4ICD (E4ICD1 or E4ICD2 generated from the respective isoforms) is translocated to the nucleus. Significantly more E4ICD2 than E4ICD1 is found in the nucleus. E4ICD2 colocalizes with YAP1 in the nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 4ICD antibody
      • ALS19 antibody
      • Avian erythroblastic leukemia viral oncogene homolog 4 antibody
      • Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody
      • E4ICD antibody
      • EC 2.7.10.1 antibody
      • ErbB 4 antibody
      • Erbb4 antibody
      • ERBB4 intracellular domain antibody
      • ERBB4_HUMAN antibody
      • HER 4 antibody
      • HER4 antibody
      • human epidermal growth factor receptor 4 antibody
      • Mer4 antibody
      • MGC138404 antibody
      • Oncogene ERBB4 antibody
      • p180erbB4 antibody
      • Proto-oncogene-like protein c-ErbB-4 antibody
      • Receptor protein tyrosine kinase erbB 4 precursor antibody
      • Receptor tyrosine protein kinase erbB 4 antibody
      • s80HER4 antibody
      • Tyrosine kinase type cell surface receptor HER4 antibody
      • Tyrosine kinase-type cell surface receptor HER4 antibody
      • v erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody
      • v erb a erythroblastic leukemia viral oncogene homolog 4 antibody
      • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) antibody
      • V-ERB-B2 avian erythroblastic leukemia viral oncogene homolog 4 antibody
      • Verba avian erythroblastic leukemia viral oncogene homolog like 4 antibody
      • Verba erythroblastic leukemia viral oncogene homolog 4 antibody
      • VERBB2 antibody
      see all

    画像

    • Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
      Western blot - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
      All lanes : Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478) at 1/5000 dilution

      Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
      Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100 ng/ml EGF for 30 min whole cell lysate
      Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with 100 ng/ml EGF for 30 min whole cell lysate, then membrane treated with Alkaline phosphatase

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 200 kDa
      Observed band size: 200 kDa


      Exposure time: 10 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB4 / HER4 with ab68478 at 1/1000 dilution, followed by ab209101 Rabbit specific IHC polymer detection kit HRP/DAB. Positive staining on Human breast carcinoma without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.

    • Immunoprecipitation - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
      Immunoprecipitation - Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)

      ErbB4 / HER4 (phospho Y1162) was immunoprecipitated from 0.35 mg of A431 treated with EGF (100ng/ml 30min) whole cell lysate with ab68478 at 1/20 dilution. Western blot was performed from the immunoprecipitate using ab68478 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

      Lane 1: A431 treated with EGF (100ng/ml 30min) whole cell lysate.
      Lane 2: ab68478 IP in A431 treated with EGF (100ng/ml 30min) whole cell lysate.
      Lane 3: Isotype control instead of ab68478 in A431 treated with EGF (100ng/ml 30min) whole cell lysate.

    • Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)
      Anti-ErbB4 / HER4 (phospho Y1162) antibody [EP2270Y] (ab68478)

    参考文献 (3)

    ab68478 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab68478 は 3 報の論文で使用されています。

    • Yoshizawa R  et al. Biphasic spatiotemporal regulation of GRB2 dynamics by p52SHC for transient RAS activation. Biophys Physicobiol 18:1-12 (2021). PubMed: 33665062
    • Yoshizawa R  et al. p52Shc regulates the sustainability of ERK activation in a RAF-independent manner. Mol Biol Cell 32:1838-1848 (2021). PubMed: 34260260
    • Ranganathan S  et al. Loss of EGFR-ASAP1 signaling in metastatic and unresectable hepatoblastoma. Sci Rep 6:38347 (2016). IHC-P ; Human . PubMed: 27910913

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ErbB 4 (phospho Y1162) antibody [EP2270Y]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Breast cancer cell lines)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9
    Permeabilization
    No
    Specification
    Breast cancer cell lines
    Blocking step
    Biocare Mach 1 Kit Blocker as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Malcolm Lim

    Verified customer

    投稿 Jul 14 2017

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