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  1. Link

    products/primary-antibodies/erbb3--her3-phospho-y1289-antibody-epr2325-ab76469.pdf

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Signal Transduction Signaling Pathway G Protein Signaling Heterotrimeric G Proteins G Proteins
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リコンビナントRabMAb

Anti-ErbB3 / HER3 (phospho Y1289) 抗体 [EPR2325] (ab76469)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (5)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
  • Western blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
  • Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2325] to ErbB3 / HER3 (phospho Y1289)
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

こちらの製品もご検討ください

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一次抗体
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Anti-ST3Gal I antibody (ab96129)

関連製品

製品の概要

  • 製品名

    Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325]
    ErbB3 / HER3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR2325] to ErbB3 / HER3 (phospho Y1289)
  • 由来種

    Rabbit
  • 特異性

    This antibody only detects ErbB 3 phosphorylated on Tyrosine 1289.

  • アプリケーション

    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • SKBR3 cell lysate treated with Neuregulin. MCF7 cells.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR2325
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Heterotrimeric G Proteins
    • G Proteins
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Growth Factors/Hormones
    • EGF
    • Cancer
    • Growth factors
    • EGF
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cancer
    • Tumor biomarkers
    • Oncoproteins
    • Neuroscience
    • Development

関連製品

  • Alternative Versions

    • Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] - BSA and Azide free (ab157376)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76469の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2500 - 1/5000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).
ICC/IF
1/100.
特記事項
WB
1/2500 - 1/5000. Detects a band of approximately 185 kDa (predicted molecular weight: 148 kDa).
ICC/IF
1/100.
追加情報
Is unsuitable for Flow Cyt,IHC-P or IP.

ターゲット情報

  • 機能

    Binds and is activated by neuregulins and NTAK.
  • 組織特異性

    Epithelial tissues and brain.
  • 関連疾患

    Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology.
  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
    Contains 1 protein kinase domain.
  • 発生段階

    Overexpressed in a subset of human mammary tumors.
  • ドメイン

    The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins.
  • 翻訳後修飾

    Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase.
  • 細胞内局在

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P21860 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2065 Human
    • Omim: 190151 Human
    • SwissProt: P21860 Human
    • Unigene: 118681 Human
    • 別名

      • c erbB 3 antibody
      • c erbB3 antibody
      • Erb b2 receptor tyrosine kinase 3 antibody
      • ErbB 3 antibody
      • ERBB3 antibody
      • ERBB3 protein antibody
      • erbB3 S antibody
      • ERBB3_HUMAN antibody
      • Glial growth factor receptor antibody
      • HER 3 antibody
      • HER3 antibody
      • Human epidermal growth factor receptor 3 antibody
      • LCCS2 antibody
      • MDA BF 1 antibody
      • MGC88033 antibody
      • p180 ErbB3 antibody
      • p45 sErbB3 antibody
      • p85 sErbB3 antibody
      • proto-oncogene-like protein c ErbB 3 antibody
      • proto-oncogene-like protein c ErbB3 antibody
      • Proto-oncogene-like protein c-ErbB-3 antibody
      • Receptor tyrosine protein kinase erbB 3 antibody
      • Receptor tyrosine protein kinase erbB3 antibody
      • Receptor tyrosine-protein kinase erbB-3 antibody
      • Tyrosine kinase type cell surface receptor HER3 antibody
      • Tyrosine kinase-type cell surface receptor HER3 antibody
      • v erb b2 avian erythroblastic leukemia viral oncogene homolog 3 antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 (avian) antibody
      • v erb b2 erythroblastic leukemia viral oncogene homolog 3 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
      Immunocytochemistry/ Immunofluorescence - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
      Immunofluorescent staining of MCF7 cells using ab76469 at 1/100 dilution.
    • Western blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
      Western blot - Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
      All lanes : Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469) at 1/5000 dilution

      Lane 1 : SKBR3 cell lysate untreated
      Lane 2 : SKBR3 cell lysate treated with Neuregulin

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 148 kDa
      Observed band size: 185 kDa why is the actual band size different from the predicted?

    • Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)
      Anti-ErbB3 / HER3 (phospho Y1289) antibody [EPR2325] (ab76469)

    プロトコール

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (5)

    ab76469 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76469 は 5 報の論文で使用されています。

    • Pereira PMR  et al. HER2-Targeted PET Imaging and Therapy of Hyaluronan-Masked HER2-Overexpressing Breast Cancer. Mol Pharm 17:327-337 (2020). PubMed: 31804840
    • Jacobi N  et al. Organotypic three-dimensional cancer cell cultures mirror drug responsesin vivo: lessons learned from the inhibition of EGFR signaling. Oncotarget 8:107423-107440 (2017). PubMed: 29296175
    • Humtsoe JO  et al. ErbB3 upregulation by the HNSCC 3D microenvironment modulates cell survival and growth. Oncogene 35:1554-64 (2016). PubMed: 26073080
    • Sollome JJ  et al. HER2/HER3 regulates extracellular acidification and cell migration through MTK1 (MEKK4). Cell Signal 26:70-82 (2013). Human . PubMed: 24036211
    • Munk M  et al. Hypoxia changes the expression of the epidermal growth factor (EGF) system in human hearts and cultured cardiomyocytes. PLoS One 7:e40243 (2012). WB ; Mouse . PubMed: 22792252

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    The tissue we will be staining is human skin.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 15 2012

    Answer

    ab51044
    -Note yet tested on frozen sections but has been tested on paraffin embedded sections
    fixation methods: formalin
    Recommended starting dilution: 1:50
    Positive control: testis, brain
    Negative control: protein is widely expressed so non-expressing tissue may be hard to find. Other controls are isotype control staining experiment, for example replacing ab51044 with Rabbit polyclonal IgG (ab27478)

    ab62435
    -Note yet tested on frozen sections
    fixation methods: 4% PFA tested in ICC only
    Recommended starting dilution:1:200
    Positive control: not yet tested in application, literature search may be helpful
    Negative control: not yet tested in application, literature search may be helpful, can also consider isotype control as noted above

    ab76469
    -Note yet tested on frozen sections
    fixation methods: 4% PFA tested in ICC only
    Recommended starting dilution: 1:100
    Positive control: not yet tested in application, literature search may be helpful
    Negative control: not yet tested in application, literature search may be helpful, can also consider isotype control as noted above

    ab75873
    -Note yet tested on frozen sections
    fixation methods: 4% PFA tested in ICC only
    Recommended starting dilution: 1:100
    Positive control: not yet tested in application, literature search may be helpful
    Negative control: not yet tested in application, literature search may be helpful, can also consider isotype control as noted above

    Since none of these 4 antibodies have yet been tested in IHC-Fr, we can not guarantee their utility in this application. However, we do have a testing discount promotion which you may be interested in. Briefly, if you buy any of these antibodies, test in IHC-Fr and provide a brief review of your procedures and results using our Abreview system (https://www.abcam.com/abreviews), this will activate a discount code I’ll provide which can then be used to get 1 free primary antibody per code. Please see below for a more detailed description of the promotion using ab51044 as an example.

    ==

    Thank you very much for your interest in ab51044.

    To our knowledge, ab/xxx/ has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab51044 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab51044 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab51044 so please wait for my reply before ordering.

    2. Purchase ab51044 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-Fr.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab51044 turns out to be unsuitable for IHC-Fr you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Feb 15 2012

    Question

    Do you have a fluorescence conjugated version?

    Read More

    Abcam community

    Verified customer

    Asked on May 09 2011

    Answer

    Thank you for your enquiry. Unfortunately, we do not have a fluorescence conjugated version of this product. Also, a custom BSA-free production is not available.

    Read More

    Abcam Scientific Support

    Answered on May 09 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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