Anti-ErbB3 / HER3 抗体 (ab34641)
Key features and details
- Rabbit polyclonal to ErbB3 / HER3
- Suitable for: IP, ELISA, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ErbB3 / HER3 antibody
ErbB3 / HER3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ErbB3 / HER3 -
由来種
Rabbit -
特異性
The antibody is directed against human HER3 and is useful in determining its presence in immunoprecipitation experiments. This antibody can detect HER3 from human, mouse and rat sources. Reactivity of this antibody with HER3 from other species is unknown.
-
アプリケーション
適用あり: IP, ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Fusion protein corresponding to Human ErbB3/ HER3 aa 1250-1350. ErbB3/ HER3 fusion protein: PASEQGYE E MRAFQGPGH QAPHVHYARL KTLRSLEAT D SAF, corresponding to C terminal amino acids 1283-1323 of Human ErbB3/ HER3
Database link: P21860 -
ポジティブ･コントロール
- IP: LNCap prostate cell line treated with Heregulin-beta1.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Rabbit Anti-HER3 is an IgG fraction antibody purified from monospecific antiserum by Protein A chromatography followed by cross adsorption against GST and extensive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34641の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
Use 5µg. The immunoprecipitation mix contained the antibody, 25 µl of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein). |
|
| ELISA |
1/10000 - 1/50000.
|
|
| WB |
1/500 - 1/2000. Predicted molecular weight: 148 kDa.
|
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. Use 5µg. The immunoprecipitation mix contained the antibody, 25 µl of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein). |
|
ELISA
1/10000 - 1/50000. |
|
WB
1/500 - 1/2000. Predicted molecular weight: 148 kDa. |
ターゲット情報
-
機能
Binds and is activated by neuregulins and NTAK. -
組織特異性
Epithelial tissues and brain. -
関連疾患
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
発生段階
Overexpressed in a subset of human mammary tumors. -
ドメイン
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
翻訳後修飾
Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 2065 Human
- Omim: 190151 Human
- SwissProt: P21860 Human
- Unigene: 118681 Human
-
別名
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
画像
-
Immunoprecipitation - Anti-ErbB3 / HER3 antibody (ab34641)All lanes : Anti-ErbB3 / HER3 antibody (ab34641) (used at 5ul for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody)
Lane 1 : LNCap prostate cell line no treatment
Lane 2 : LNCap prostate cell line treated with Heregulin beta1 for 15mins at 100ng/ml
Developed using the ECL technique.
Observed band size: 180 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab34641 は 3 報の論文で使用されています。
- Kim H et al. ErbB3, a possible prognostic factor of head and neck squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol 129:377-387 (2020). PubMed: 32081558
- Poitelon Y et al. Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Nat Commun 6:8303 (2015). WB ; Rat . PubMed: 26383514
- Yan X et al. miR-143 and miR-145 synergistically regulate ERBB3 to suppress cell proliferation and invasion in breast cancer. Mol Cancer 13:220 (2014). WB ; Human . PubMed: 25248370