Anti-ErbB2 / HER2 抗体 [SP3] (ab16662)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)](https://www.abcam.co.jp/ps/products/16/ab16662/Images/ab16662-6-antierbb2her2-antibody-sp3-breast-cancer-human-ventana.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP3] to ErbB2 / HER2
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ErbB2 / HER2 antibody [SP3]
ErbB2 / HER2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP3] to ErbB2 / HER2 -
由来種
Rabbit -
特異性
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アプリケーション
適用あり: IHC-Pmore details
適用なし: Flow Cyt,ICC,IHC-Fr,IP or WB -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Recombinant fragment. This information is considered to be commercially sensitive.
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エピトープ
Extracellular domain -
ポジティブ･コントロール
- IHC-P: Human breast carcinoma tissue.
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特記事項
This product was switched from a hybridoma to recombinant production method on 5th March 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SP3 -
アイソタイプ
IgG -
研究分野
関連製品
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Affibody® Molecule
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16662の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab16662 is not suitable for [IHC-P, Mouse] |
| 特記事項 |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. ab16662 is not suitable for [IHC-P, Mouse] |
ターゲット情報
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機能
Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. -
組織特異性
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. -
関連疾患
Hereditary diffuse gastric cancer
Glioma
Ovarian cancer
Lung cancer
Gastric cancer
Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172). -
細胞内局在
Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. - Information by UniProt
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参照データベース
- Entrez Gene: 2064 Human
- Omim: 164870 Human
- SwissProt: P04626 Human
- Unigene: 446352 Human
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別名
- Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody
- C erb B2/neu protein antibody
- CD340 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)
Immunohistochemical analysis of formalin fixed paraffin embedded human breast cancer labelling ErbB2 with ab16662 at a concentration of 0.16 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab16662 anti-ErbB2 / HER2 antibody [SP3] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)](https://www.abcam.co.jp/ps/products/16/ab16662/Images/ab16662-323690-anti-erbb-2-antibody-sp3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)This image is courtesy of an Abreview submitted by Mr. Allan Wang.Formalin-fixed, paraffin-embedded human breast invasive ductal carcinoma tissue stained for ErbB2 / HER2 using ab16662 at 1/200 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG HRP. Heat mediated antigen retrieval was done using a pH 8.5 buffer.
This image was generated from the hybridoma version.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)](https://www.abcam.co.jp/ps/products/16/ab16662/Images/ab16662-356824-anti-erbb-2-antibody-sp3-immunohistochemistry-breast-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [SP3] (ab16662)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling ErbB 2 with ab16662, followed by a ready to use Goat Anti-Rabbit & Mouse IgG (HRP). Positive staining on Human breast carcinoma, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab16662 for 10 mins at room temperature. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 10 minutes. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit & Mouse IgG (HRP).
This image was generated from the hybridoma version.
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![Anti-ErbB2 / HER2 antibody [SP3] (ab16662)](https://www.abcam.co.jp/ps/products/16/ab16662/Images/ab16662-4-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [SP3] (ab16662)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab16662 は 8 報の論文で使用されています。
- Arévalo AP et al. In Vivo Evaluation of Sgc8-c Aptamer as a Molecular Imaging Probe for Colon Cancer in a Mouse Xenograft Model. Int J Mol Sci 23:N/A (2022). PubMed: 35269608
- Liu Y et al. Combining Organoid Models with Next-Generation Sequencing to Reveal Tumor Heterogeneity and Predict Therapeutic Response in Breast Cancer. J Oncol 2022:9390912 (2022). PubMed: 36046364
- Yeo MK et al. HER2 Expression in Peritoneal Dissemination of High-Grade Serous Ovarian Carcinoma: A Comparative Study of Immunohistochemical Reactivity Using Four HER2 Antibodies. J Clin Med 11:N/A (2022). PubMed: 36498537
- Park J et al. Genetic profiling of somatic alterations by Oncomine Focus Assay in Korean patients with advanced gastric cancer. Oncol Lett 20:129 (2020). PubMed: 32934698
- Jin X et al. ERa is required for suppressing OCT4-induced proliferation of breast cancer cells via DNMT1/ISL1/ERK axis. Cell Prolif 52:e12612 (2019). PubMed: 31012189
- Tian M & Schiemann WP TGF-ß Stimulation of EMT Programs Elicits Non-genomic ER-a Activity and Anti-estrogen Resistance in Breast Cancer Cells. J Cancer Metastasis Treat 3:150-160 (2017). PubMed: 28955730
- Park SY et al. Does background parenchymal enhancement on MRI affect the rate of positive resection margin in breast cancer patients? Br J Radiol 88:20140638 (2015). PubMed: 25429418
- Demir L et al. Hormone receptor, HER2/NEU and EGFR expression in ovarian carcinoma--is here a prognostic phenotype? Asian Pac J Cancer Prev 15:9739-45 (2014). PubMed: 25520097