AB192868

Anti-ERAS 抗体 [EPR15595]

Anti-ERAS antibody [EPR15595]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal ERAS antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications.

別名を表示する

HRAS2, HRASP, ERAS, GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras

2 Images

Supplier Data

Western blot - Anti-ERAS antibody [EPR15595] (AB192868)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ERAS antibody [EPR15595] (ab192868) at 1/5000 dilution

All lanes:

F9 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

Supplier Data

Western blot - Anti-ERAS antibody [EPR15595] (AB192868)

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ERAS antibody [EPR15595] (ab192868) at 1/1000 dilution

All lanes:

Recombinant Human ERAS protein (ab158695) at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 25 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15595

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ERAS also known as Embryonic Stem Cell Ras (EC-Ras) is a member of the Ras protein family. It has a molecular weight of approximately 23 kDa. ERAS is mainly expressed in embryonic stem cells and specific cancer cells. It shares structural similarities with other Ras family proteins having a low GTPase activity that contributes to its role as a molecular switch in signaling pathways. Like other Ras proteins ERAS toggles between active GTP-bound and inactive GDP-bound forms which influences downstream signaling pathways.

Biological function summary

The ERAS protein influences cell proliferation survival and growth in tissues where it is expressed. It is not part of a larger protein complex but rather acts independently to stimulate the phosphoinositide 3-kinase (PI3K) pathway. This activation of the PI3K pathway leads to the downstream activation of Akt a critical effector in cellular growth and survival processes. By promoting these cellular functions ERAS contributes to maintaining the self-renewal properties of stem cells and influencing oncogenic processes in cancer cells.

Pathways

ERAS engages actively with the PI3K/Akt signaling pathway facilitating the survival and growth of cells. It directly interacts with the PI3K protein promoting cellular proliferation. Additionally ERAS has interactions with the MAPK/ERK pathway a well-known pathway linked to cell division and differentiation. The interaction between these pathways involves proteins like Raf and MEK which integrates growth signals coming from activated Ras proteins.

ERAS expression has been associated with certain types of cancers notably germ cell tumors and possibly breast cancer. Through its interaction with the PI3K/Akt signaling pathway ERAS contributes to the oncogenic properties observed in these cancers. Moreover its activities can influence the behavior of proteins such as PTEN and p53 which are well known for their tumor suppressor functions. The modulation of these pathways and interactions ultimately affect cellular processes like apoptosis which can contribute to the development and progression of cancer.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells (By similarity).

See full target information ERAS

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39146021

2024

PRMT5-mediated FUBP1 methylation accelerates prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Yan,Xun Liu,Xuefeng Qiu,Xuebin Zhang,Jiahui Chen,Kai Xiao,Ping Wu,Chao Peng,Xiaolin Hu,Zengming Wang,Jun Qin,Liming Sun,Luonan Chen,Denglong Wu,Shengsong Huang,Lichen Yin,Zhenfei Li

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34921114

2021

N6-methyladenosine (mA) depletion regulates pluripotency exit by activating signaling pathways in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kang-Xuan Jin,Rujuan Zuo,Konstantinos Anastassiadis,Arne Klungland,Carsten Marr,Adam Filipczyk

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERAS 抗体 [EPR15595]

Anti-ERAS antibody [EPR15595]

Western blot - Anti-ERAS antibody [EPR15595] (AB192868)

Western blot - Anti-ERAS antibody [EPR15595] (AB192868)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (2)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise