Anti-EPS15R 抗体 [EP1147Y] (ab53006)
![Western blot - Anti-EPS15R antibody [EP1147Y] (ab53006)](https://www.abcam.co.jp/ps/products/53/ab53006/Images/ab53006-65598-anti-eps15r-antibody-ep1147y-western-blot.jpg "Western blot - Anti-EPS15R antibody [EP1147Y] (ab53006)")
Key features and details
- Rabbit monoclonal [EP1147Y] to EPS15R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-EPS15R antibody [EP1147Y]
EPS15R 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1147Y] to EPS15R -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HeLa cell lysate. IHC-P: Human breast carcinoma tissue.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP1147Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53006の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa).
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/50000. Detects a band of approximately 125 kDa (predicted molecular weight: 94 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. -
配列類似性
Contains 1 EF-hand domain.
Contains 3 EH domains. -
翻訳後修飾
Phosphorylated on tyrosine residues by EGFR. -
細胞内局在
Cell membrane. Nucleus. Membrane > coated pit. Localized to plasma membrane coated pits. - Information by UniProt
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参照データベース
- Entrez Gene: 58513 Human
- Omim: 600051 Human
- SwissProt: Q9UBC2 Human
- Unigene: 654639 Human
- Unigene: 744842 Human
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別名
- EP15R_HUMAN antibody
- epidermal growth factor receptor pathway substrate 15- ike 1 antibody
- Epidermal growth factor receptor substrate 15 like 1 antibody
see all
画像
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Western blot - Anti-EPS15R antibody [EP1147Y] (ab53006)Anti-EPS15R antibody [EP1147Y] (ab53006) at 1/50000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 94 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS15R antibody [EP1147Y] (ab53006)](https://www.abcam.co.jp/ps/products/53/ab53006/Images/ab53006-65597-anti-eps15r-antibody-ep1147y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPS15R antibody [EP1147Y] (ab53006)ab53006, at a 1/50 dilution, staining EPS15R in human breast carcinoma, using Immunohistochemsitry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab53006 は 6 報の論文で使用されています。
- Séraudie I et al. A new scaffold-free tumoroid model provides a robust preclinical tool to investigate invasion and drug response in Renal Cell Carcinoma. Cell Death Dis 14:622 (2023). PubMed: 37736770
- Day KJ et al. Liquid-like protein interactions catalyse assembly of endocytic vesicles. Nat Cell Biol 23:366-376 (2021). PubMed: 33820972
- Traub LM A nanobody-based molecular toolkit provides new mechanistic insight into clathrin-coat initiation. Elife 8:N/A (2019). PubMed: 31038455
- Evergren E et al. Eps15R and clathrin regulate EphB2-mediated cell repulsion. Traffic 19:44-57 (2018). PubMed: 28972287
- Liu Z & Zheng Y A requirement for epsin in mitotic membrane and spindle organization. J Cell Biol 186:473-80 (2009). PubMed: 19704019
- Chen C & Zhuang X Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Proc Natl Acad Sci U S A 105:11790-5 (2008). PubMed: 18689690