Anti-EPPIN 抗体 (ab185699)
Key features and details
- Rabbit polyclonal to EPPIN
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EPPIN antibody -
製品の詳細
Rabbit polyclonal to EPPIN -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human EPPIN aa 22-133. Mature form.
Database link: O95925 -
ポジティブ･コントロール
- THP1, Mouse liver and Mouse testis lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.09% Sodium azide
Constituents: 49.91% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 15 kDa.
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| IHC-P |
1/50 - 1/200.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 15 kDa. |
|
IHC-P
1/50 - 1/200. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Serine protease inhibitor that plays an essential role in male reproduction and fertility. Modulates the hydrolysis of SEMG1 by KLK3/PSA (a serine protease), provides antimicrobial protection for spermatozoa in the ejaculate coagulum, and binds SEMG1 thereby inhibiting sperm motility. -
組織特異性
In testis, expressed and secreted by Sertoli cells, appearing on the surface of testicular and ejaculate spermatozoa. Expressed in the spermatogonia and the earliest preleptotene spermatocytes. In the epididymis, is expressed and secreted by epithelial cells and covers the surface of epididymal spermatozoa and ciliated epithelial cells (at protein level). Expressed specifically in epididymis and testis. Isoform 2 is expressed only in the epididymis. Weak expression is detected in myoid cells as well as spermatogenic cells. -
配列類似性
Contains 1 BPTI/Kunitz inhibitor domain.
Contains 1 WAP domain. -
ドメイン
The BPTI/Kunitz inhibitor domain is required for elastase inhibitory activity. BPTI/Kunitz inhibitor and WAP domains are involved in the protein antibacterial activity. -
細胞内局在
Secreted. Cell surface. Bound to the surface of testicular and on the head and tail of ejaculate spermatozoa. - Information by UniProt
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参照データベース
- Entrez Gene: 100526773 Human
- Entrez Gene: 57119 Human
- Entrez Gene: 75526 Mouse
- Omim: 609031 Human
- SwissProt: O95925 Human
- SwissProt: Q9DA01 Mouse
- Unigene: 121084 Human
- Unigene: 274876 Human
see all -
別名
- Cancer/testis antigen 71 antibody
- CT 71 antibody
- CT71 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPPIN antibody (ab185699)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling EPPIN with ab185699 at 1/200. Magnification: 400x.
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Immunocytochemistry/ Immunofluorescence - Anti-EPPIN antibody (ab185699)Immunocytochemistry/Immunofluorescence analysis of HepG2 cells using ab185699. Blue DAPI for nuclear staining. -
Western blot - Anti-EPPIN antibody (ab185699)All lanes : Anti-EPPIN antibody (ab185699) at 1/500 dilution
Lane 1 : THP1 cell lysate
Lane 2 : Mouse liver lysate
Lane 3 : Mouse testis lysate
Predicted band size: 15 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185699 は論文での使用が確認できていません。