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Anti-Epoxide hydrolase 抗体 (ab96695)

Submit a review Q&A (1)References (4)
Western blot - Anti-Epoxide hydrolase antibody (ab96695)
  • Immunocytochemistry/ Immunofluorescence - Anti-Epoxide hydrolase antibody (ab96695)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (ab96695)

Key features and details

  • Rabbit polyclonal to Epoxide hydrolase
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Epoxide hydrolase antibody
    Epoxide hydrolase 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Epoxide hydrolase

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig

  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1-193 of Human Epoxide hydrolase (NP_000111).

  • ポジティブ･コントロール

    • WB: 293T, HeLa and HepG2 cell lysates ICC/IF: Hela cells IHC-P: SG xenograft

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab96695の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P
1/100 - 1/500.
ICC/IF
1/100 - 1/200.
特記事項
WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
IHC-P
1/100 - 1/500.
ICC/IF
1/100 - 1/200.

ターゲット情報

  • 機能

    Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.

  • 組織特異性

    Found in liver.

  • 関連疾患

    Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
    Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.

  • 配列類似性

    Belongs to the peptidase S33 family.

  • 細胞内局在

    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • EPHX 1 antibody
    • EPHX antibody
    • Ephx1 antibody
    • EPOX antibody
    • Epoxide hydratase antibody
    • Epoxide hydrolase 1 antibody
    • Epoxide hydrolase 1 microsomal (xenobiotic) antibody
    • Epoxide hydroxylase 1 antibody
    • Epoxide hydroxylase 1 microsomal (xenobiotic) antibody
    • HYEP_HUMAN antibody
    • HYL1 antibody
    • MEH antibody
    • Microsomal epoxide hydrolase antibody
    see all

画像

  • Western blot - Anti-Epoxide hydrolase antibody (ab96695)
    Western blot - Anti-Epoxide hydrolase antibody (ab96695)
    All lanes : Anti-Epoxide hydrolase antibody (ab96695) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Epoxide hydrolase antibody (ab96695)
    Immunocytochemistry/ Immunofluorescence - Anti-Epoxide hydrolase antibody (ab96695)
    ab96695, at a 1/200 dilution, staining Epoxide hydrolase in methanol fixed HeLa cells by Immunofluorescence analysis. Lower image costained with Hoerchst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (ab96695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Epoxide hydrolase antibody (ab96695)
    ab96695, at a 1/500 dilution, staining Epoxide hydrolase in paraffin embedded SG xenograft by Immunohistochemical analysis.

参考文献 (4)

ab96695 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab96695 は 4 報の論文で使用されています。

  • González-Alvarez ME  et al. Obesity alters the ovarian proteomic response to zearalenone exposure†. Biol Reprod 105:278-289 (2021). PubMed: 33855340
  • Cheng H  et al. Emerging role of EPHX1 in chemoresistance of acute myeloid leukemia by regurlating drug-metabolizing enzymes and apoptotic signaling. Mol Carcinog N/A:N/A (2019). PubMed: 30644597
  • Kumar M  et al. Insulin activates intracellular transport of lipid droplets to release triglycerides from the liver. J Cell Biol 218:3697-3713 (2019). PubMed: 31604801
  • O'Shaughnessy PJ  et al. Maternal smoking and fetal sex significantly affect metabolic enzyme expression in the human fetal liver. J Clin Endocrinol Metab 96:2851-60 (2011). WB ; Human . PubMed: 21715529

レビューと Q&A

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レビューを送る 質問を送る

Question

I would like to test this antibody in WB using samples from mouse and rat, which are untested species.

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Answer

Thank you very much for your interest in ab96695. To our knowledge, ab96695 has not been tested in mouse or rat . Therefore, I can offer a discount off a future purchase if you buy ab96695 now, test it in mouse or rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab96695 in mouse and rat. I will then send a discount code. This code must be issued before purchasing ab96695 so please wait for my reply before ordering. 2. Purchase ab96995 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse or rat . 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab96695 turns out to be unsuitable for mouse or rat, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

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