Anti-Ephexin-1 抗体 - C-terminal (ab157593)
Key features and details
- Rabbit polyclonal to Ephexin-1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Ephexin-1 antibody - C-terminal
Ephexin-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ephexin-1 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken
-
免疫原
Synthetic peptide coupled to a carrier protein, corresponding to C-terminal amino acids of Mouse Ephexin-1 (UniProt: Q8CHT1).
-
ポジティブ･コントロール
- A431, Jurkat and HUVEC cell lysates; Rat PC12 cell lysate; Mouse brain tissue lysate; Mouse C2C12 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab157593の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 82 kDa.
|
| ICC/IF |
1/50.
|
| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 82 kDa. |
|
ICC/IF
1/50. |
ターゲット情報
-
機能
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension. -
組織特異性
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. -
配列類似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain. -
ドメイン
The DH domain and the PH domain are both required to mediate interaction with EPHA4. -
翻訳後修飾
Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. -
細胞内局在
Cytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones. - Information by UniProt
-
参照データベース
- Entrez Gene: 424744 Chicken
- Entrez Gene: 25791 Human
- Entrez Gene: 53972 Mouse
- Entrez Gene: 246217 Rat
- Omim: 605991 Human
- SwissProt: Q8N5V2 Human
- SwissProt: Q8CHT1 Mouse
- SwissProt: Q5BKC9 Rat
see all -
別名
- ARHGEF27 antibody
- Eph receptor-interacting exchange protein antibody
- Eph-interacting exchange protein antibody
see all
画像
-
Western blot - Anti-Ephexin-1 antibody - C-terminal (ab157593)All lanes : Anti-Ephexin-1 antibody - C-terminal (ab157593) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A431 cell lysate, treated with pervanadate (1mM; 30 mins)
Predicted band size: 82 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Ephexin-1 antibody - C-terminal (ab157593)Immunofluorescent analysis of Mouse C2C12 cells, labeling Ephexin-1 with ab157593 at 1/50 dilution. Right image shows cells treated with pervanadate.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab157593 は論文での使用が確認できていません。