Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) 抗体 (ab61791)
Key features and details
- Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody
Eph receptor B1 + Eph receptor B2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) -
由来種
Rabbit -
特異性
ab61791 detects endogenous levels of EPHB1/2 only when phosphorylated at tyrosine 594 and tyrosine 596.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596).
Database link: P54763-3 -
ポジティブ･コントロール
- Extracts from HepG2 cells, HuvEc cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61791の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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| ICC/IF | (2) |
1/500 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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関連性
Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor. -
細胞内局在
Membrane; Single-pass type I membrane protein. -
参照データベース
- Entrez Gene: 2047 Human
- Entrez Gene: 2048 Human
- Entrez Gene: 13844 Mouse
- Entrez Gene: 270190 Mouse
- Entrez Gene: 24338 Rat
- Entrez Gene: 313633 Rat
- Omim: 600600 Human
- Omim: 600997 Human
see all -
別名
- EPH B1 antibody
- EPH B2 antibody
- Eph tyrosine kinase 2 antibody
see all
画像
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Western blot - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)This image is courtesy of an Abreview submitted by Thomas JungasAll lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate - overexpressing EphB2
Lane 3 : HeLa whole cell lysate - overexpressinf EphB2-kinase dead (K661R)
Lysates/proteins at 100000 cells per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 5 minutesAll cells were stimulated with preclustered ephrinb1-Fc chimeric protein.
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Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)This image is courtesy of an Abreview submitted by Thomas Jungasab61791 staining Eph receptor B1 + Eph receptor B2 (phospho Y594) in HeLa cells (overexpressing EphB2 and stimulated for 20 minutes with preclustered efnb1-Fc chimeric protein) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 0.1% Triton X-100) for 2 hour at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody. Red - anti-EphB2 (goat), Grey - DAPI, Green - ab61791.
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Western blot - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells
Lane 2 : Extracts from HepG2 cells with the immunising phosphopeptide
Predicted band size: 110 kDa
Observed band size: 110 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y596) antibody (ab61791)Immunofluorescence analysis of HuvEc cells, using ab61791 at 1/500-1/1000 dilution with (+) or without (-) immunising phosphopeptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab61791 は 23 報の論文で使用されています。
- Yao Z et al. Age-related decline in hippocampal tyrosine phosphatase PTPRO is a mechanistic factor in chemotherapy-related cognitive impairment. JCI Insight 8:N/A (2023). PubMed: 37485875
- Lewis AE et al. Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting. Cell Rep 38:110510 (2022). PubMed: 35294885
- Wang L et al. Cell-cell contact-driven EphB1 cis- and trans- signalings regulate cancer stem cells enrichment after chemotherapy. Cell Death Dis 13:980 (2022). PubMed: 36402751
- Huang Z et al. Key role for EphB2 receptor in kidney fibrosis. Clin Sci (Lond) 135:2127-2142 (2021). PubMed: 34462781
- Ahmed MS et al. Identification of tetracycline combinations as EphB1 tyrosine kinase inhibitors for treatment of neuropathic pain. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33627480