Anti-Eph receptor B1 + Eph receptor B2 抗体 (ab61765)
Key features and details
- Rabbit polyclonal to Eph receptor B1 + Eph receptor B2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Eph receptor B1 + Eph receptor B2 antibody
Eph receptor B1 + Eph receptor B2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic non-phosphopeptide derived from human Eph receptor B1 from around the phosphorylation site of tyrosine 594 (K-I-YP-I-D).
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ポジティブ･コントロール
- Extracts from HepG2 cells, HuvEc cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab61765の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/50 - 1/100.
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| WB |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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| ICC/IF |
1/500 - 1/1000.
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| 特記事項 |
|---|
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IHC-P
1/50 - 1/100. |
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WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
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ICC/IF
1/500 - 1/1000. |
ターゲット情報
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関連性
Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor. -
細胞内局在
Membrane; Single-pass type I membrane protein. -
参照データベース
- Entrez Gene: 2047 Human
- Entrez Gene: 2048 Human
- Entrez Gene: 13844 Mouse
- Entrez Gene: 270190 Mouse
- Entrez Gene: 24338 Rat
- Entrez Gene: 313633 Rat
- Omim: 600600 Human
- Omim: 600997 Human
see all -
別名
- EPH B1 antibody
- EPH B2 antibody
- Eph tyrosine kinase 2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 + Eph receptor B2 antibody (ab61765)HuvEc cells stained wth ab61765 at 1/500 - 1/1000, with and without the immunising peptide.
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Western blot - Anti-Eph receptor B1 + Eph receptor B2 antibody (ab61765)All lanes : Anti-Eph receptor B1 + Eph receptor B2 antibody (ab61765) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells.
Lane 2 : Extracts from HepG2 cells and the immunising peptide.
Predicted band size: 110 kDa
Observed band size: 110 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B1 + Eph receptor B2 antibody (ab61765)Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EPHB1/2 Antibody. The picture on the right is blocked with the synthesized peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab61765 は 1 報の論文で使用されています。
- Li L et al. Prognostic values of EphB1/B2 and p-EphB1/B2 expression in non-small cell lung cancer. Int J Clin Exp Pathol 10:10092-10101 (2017). PubMed: 31966900