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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Eph receptor A2 抗体 [EPR24711-11] - BSA and Azide free (ab290755)

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Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)
  • Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)
  • Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24711-11] to Eph receptor A2 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free
    Eph receptor A2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24711-11] to Eph receptor A2 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate, K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate, Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate, PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate, A549 (human lung carcinoma epithelial cell) whole cell lysate

  • 特記事項

    ab290755 is the carrier-free version of ab273118

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab290755の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.

  • 組織特異性

    Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.

  • 関連疾患

    Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.

  • 翻訳後修飾

    Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ARCC2 antibody
      • AW545284 antibody
      • CTPA antibody
      • CTPP1 antibody
      • CTRCT6 antibody
      • EC 2.7.10.1 antibody
      • Eck antibody
      • Eph receptor A2 antibody
      • EPHA2 antibody
      • EPHA2_HUMAN antibody
      • Ephrin receptor antibody
      • Ephrin receptor EphA2 antibody
      • Ephrin type A receptor 2 antibody
      • Ephrin type-A receptor 2 antibody
      • Epithelial cell kinase antibody
      • Epithelial cell receptor protein tyrosine kinase antibody
      • Myk 2 antibody
      • Myk2 antibody
      • Sek 2 antibody
      • Sek2 antibody
      • Soluble EPHA2 variant 1 antibody
      • Tyrosine protein kinase receptor ECK antibody
      • Tyrosine-protein kinase receptor ECK antibody
      • Tyrosine-protein kinase receptor MPK-5 antibody
      • Tyrosine-protein kinase receptor SEK-2 antibody
      see all

    画像

    • Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)
      Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)
      All lanes : Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118) at 1/1000 dilution

      Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate.
      Lane 2 : Ephrin type-A receptor 2 knockout HeLa (ab264795) whole cell lysate.
      Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate.
      Lane 4 : A549 (human lung carcinoma epithelial cell) whole cell lysate.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (ab216776) at 1/10000 dilution

      Predicted band size: 108 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?


      Exposure time: 81 seconds


      This data was developed using ab273118, the same antibody clone in a different buffer formulation. 

      Blocking / Diluent buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS

      Lanes 1-4: Merged signal (red and green). Green - ab273118 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa.

      ab273118 Anti-Ephrin type-A receptor 2 antibody [EPR24711-11] was shown to specifically react with Ephrin type-A receptor 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line (ab264795) was used. Wild-type and Ephrin type-A receptor 2 knockout samples were subjected to SDS-PAGE. ab273118 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4? overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)
      Western blot - Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)
      All lanes : Anti-Eph receptor A2 antibody [EPR24711-11] (ab273118) at 1/1000 dilution

      Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
      Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 108 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?


      Exposure time: 81 seconds


      This data was developed using ab273118, the same antibody clone in a different buffer formulation. 

      Blocking / Diluent buffer and concentration: 5% NFDM/TBST

    • Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)
      Anti-Eph receptor A2 antibody [EPR24711-11] - BSA and Azide free (ab290755)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab290755 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab290755 は論文での使用が確認できていません。

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