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リコンビナントRabMAb

Anti-EPCR/CD201 抗体 [EPR26267-44] - BSA and Azide free (ab302912)

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Western blot - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
  • Western blot - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
  • Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26267-44] to EPCR/CD201 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-EPCR/CD201 antibody [EPR26267-44] - BSA and Azide free
    EPCR/CD201 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26267-44] to EPCR/CD201 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat
    非交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Rat placenta and mouse lung tissue lysate. bEnd.3 whole cell lysate. IHC-P: Mouse kidney and rat lung tissue. bEnd.3 and B16-F10 cell pellet.

  • 特記事項

    AB302912 is a carrier free version of AB302911. 

    AB302911 does not react in: WB and IHC-P with human samples; ICC/IF, Flow Cytometry, and IP with mouse samples. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302912の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 45-55 kDa (predicted molecular weight: 27 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 45-55 kDa (predicted molecular weight: 27 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

  • 組織特異性

    Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.

  • 翻訳後修飾

    N-glycosylated.
    A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activated protein C receptor antibody
      • APC receptor antibody
      • APCR antibody
      • bA42O4.2 antibody
      • CCCA antibody
      • CCD41 antibody
      • CD201 antibody
      • CD201 antigen antibody
      • cell cycle, centrosome-associated protein antibody
      • Cell cycle, centrosome-associated protein antibody
      • centrocyclin antibody
      • Endothelial cell protein C receptor antibody
      • Endothelial protein C receptor antibody
      • EPCR antibody
      • EPCR_HUMAN antibody
      • MGC23024 antibody
      • PROCR antibody
      • Protein C receptor, endothelial antibody
      see all

    画像

    • Western blot - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      Western blot - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      All lanes : Anti-EPCR/CD201 antibody [EPR26267-44] (ab302911) at 1/1000 dilution

      Lane 1 : bEnd.3 (mouse brain endothelial cell) whole cell lysate
      Lane 2 : B16-F10 (mouse skin melanoma cell) whole cell lysate
      Lane 3 : Mouse lung tissue lysate
      Lane 4 : Mouse brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 27 kDa
      Observed band size: 45-55 kDa why is the actual band size different from the predicted?



      This data was developed using AB302911, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer: 5% NFDM/TBST. 

      Low expression: B16-F10 (PMID: 19188668); mouse brain (PMID: 10688825).

      Lanes 3-4 were developed using a high sensitivity ECL substrate.

      Exposure time: Lanes 1-2: 10 seconds;Lanes 3-4: 136 seconds.

    • Western blot - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      Western blot - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      All lanes : Anti-EPCR/CD201 antibody [EPR26267-44] (ab302911) at 1/1000 dilution

      Lane 1 : Rat placenta tissue lysate
      Lane 2 : Rat brain tissue lysate
      Lane 3 : Rat testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 27 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using AB302911, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer: 5% NFDM/TBST. 

      Low expression: brain, testis (PMID: 10688825).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)

      This data was developed using AB302911, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling EPCR/CD201 with ab302911 at 1/2000 dilution (0.266 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on endothelial cells of mouse kidney was observed. The section was incubated with ab302911 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)

      This data was developed using AB302911, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling EPCR/CD201 with ab302911 at 1/2000 dilution (0.266 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on endothelial cells of rat lung was observed. The section was incubated with ab302911 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)

      This data was developed using AB302911, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (Panel A) bEnd.3 (Mouse brain endothelioma) and (Panel B) B16-F10 (Mouse skin melanoma) cell pellets labeling EPCR/CD201 with ab302911 at 1/5000 dilution (0.106 µg/ml), followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on (Panel A) bEnd.3 cell pellet, very weak staining on (Panel B) B16-F10 cell pellet were observed. The section was incubated with ab302911 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)

      This data was developed using AB302911, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded mouse glioblastoma tissue labeling EPCR/CD201 with ab302911 at 1/2000 dilution (0.266 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). The section was incubated with ab302911 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Negative control: no staining on mouse glioblastoma was observed.

      Secondary antibody only control: A ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)
      Anti-EPCR/CD201 antibody [EPR26267-44] (BSA and Azide free) (AB302912)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302912 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302912 は論文での使用が確認できていません。

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