Anti-EPCR/CD201 抗体 [EPR26267-19] (ab300523)
![Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR26267-19] (ab300523)](https://www.abcam.co.jp/ps/products/300/ab300523/Images/AB300523-486233-anti-EPCRCD201-EPR2626719-ICC-mouse-bEnd3.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR26267-19] (ab300523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26267-19] to EPCR/CD201
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-EPCR/CD201 antibody [EPR26267-19]
EPCR/CD201 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR26267-19] to EPCR/CD201 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, Flow Cytmore details
適用なし: IHC-P,IP or WB -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: bEnd.3 cells. Flow Cyt: bEnd.3 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR26267-19 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300523の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500.
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| Flow Cyt |
1/500.
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| 特記事項 |
|---|
|
ICC/IF
1/500. |
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Flow Cyt
1/500. |
ターゲット情報
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機能
Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation. -
組織特異性
Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. -
翻訳後修飾
N-glycosylated.
A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 19124 Mouse
- SwissProt: Q64695 Mouse
- Unigene: 3243 Mouse
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別名
- Activated protein C receptor antibody
- APC receptor antibody
- APCR antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR26267-19] (ab300523)
Immunofluorescent analysis of 100% Methanol fixed, 0.1% Triton X-100 permeabilized bEnd.3 (mouse brain endothelioma endothelialpolyoma middle T antigen transformed) cells labeling EPCR/CD201 with ab300523 at 1/500 (0.988 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing cytoplasmic staining in bEnd.3 cell line and low expression in B16-F10 (murine melanoma cell line). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml) was used to label Tubulin (Red) as a counterstain. DAPI was used as a nuclear counterstain (Blue).
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![Flow Cytometry - Anti-EPCR/CD201 antibody [EPR26267-19] (ab300523)](https://www.abcam.co.jp/ps/products/300/ab300523/Images/AB300523-486232-anti-EPCRCD201-EPR2626719-flow-cytometry-mouse.jpg)
Flow Cytometry - Anti-EPCR/CD201 antibody [EPR26267-19] (ab300523)Flow Cytometry analysis of B16-F10 (Mouse skin melanoma, Left) / bEnd.3 (mouse brain endothelioma, Right) labeling EPCR/CD201 (red) with ab300523 at a 1/500 dilution. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730) Isotype control. Blue - (unlabeled control) - Cells without incubation with primary and secondary antibodies. Gated on viable cells.
Low expression: B16-F10 (PMID: 19188668).
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![Anti-EPCR/CD201 antibody [EPR26267-19] (AB300523)](https://www.abcam.co.jp/ps/products/300/ab300523/Images/AB300523-486158-Benefits-of-Recombinant-antibodies.jpg)
Anti-EPCR/CD201 antibody [EPR26267-19] (AB300523)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300523 は論文での使用が確認できていません。