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リコンビナントRabMAb

Anti-EPCR/CD201 抗体 [EPR25647-5] - BSA and Azide free (ab300526)

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Western blot - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)
  • Western blot - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (ab300526)
  • Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (ab300526)
  • Flow Cytometry - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)
  • Immunoprecipitation - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)
  • Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25647-5] to EPCR/CD201
  • Suitable for: IP, WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free
    EPCR/CD201 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25647-5] to EPCR/CD201

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HUVEC, Ea.Hy926, RKO, Human placenta and Human lung whole cell lysates. Untreated HUVEC, and HUVEC cells treated with Protein Deglycosylation Mix II whole cell lysates. ICC/IF: HUVEC cells. Flow Cyt: HUVEC cells. IP: HUVEC cell.

  • 特記事項

    ab300526 is the carrier-free version of ab300525.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300526の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P.

ターゲット情報

  • 機能

    Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

  • 組織特異性

    Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.

  • 翻訳後修飾

    N-glycosylated.
    A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activated protein C receptor antibody
      • APC receptor antibody
      • APCR antibody
      • bA42O4.2 antibody
      • CCCA antibody
      • CCD41 antibody
      • CD201 antibody
      • CD201 antigen antibody
      • cell cycle, centrosome-associated protein antibody
      • Cell cycle, centrosome-associated protein antibody
      • centrocyclin antibody
      • Endothelial cell protein C receptor antibody
      • Endothelial protein C receptor antibody
      • EPCR antibody
      • EPCR_HUMAN antibody
      • MGC23024 antibody
      • PROCR antibody
      • Protein C receptor, endothelial antibody
      see all

    画像

    • Western blot - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)
      Western blot - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)
      All lanes : Anti-EPCR/CD201 antibody [EPR25647-5] (ab300525) at 1/1000 dilution

      Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
      Lane 2 : Ea.Hy926 (human somatic cell hybrid endothelial), whole cell lysate
      Lane 3 : RKO (human colon epithelial cell), whole cell lysate
      Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
      Lane 5 : Human placenta tissue lysate
      Lane 6 : Human lung tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Observed band size: 45 kDa why is the actual band size different from the predicted?



      This data was developed using ab300525, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: Jurkat (PMID: 7929370)

      Exposure time: Lanes 1-4: 103 seconds Lanes 5-6: 3 minutes

    • Western blot - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (ab300526)
      Western blot - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (ab300526)
      All lanes : Anti-EPCR/CD201 antibody [EPR25647-5] (ab300525) at 1/1000 dilution

      All lanes : Untreated HUVEC whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Observed band size: 45 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This data was developed using ab300525, the same antibody clone in a different buffer formulation. 

      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    • Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (ab300526)
      Immunocytochemistry/ Immunofluorescence - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (ab300526)

      This data was developed using ab300525, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cells) labelling EPCR/CD201 with ab300525 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line. The nuclear counter stain is DAPI (blue).

      Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (red) at 1/200 dilution.

      Negative control: Jurkat (PMID: 11703343)

    • Flow Cytometry - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)
      Flow Cytometry - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)

      This data was developed using ab300525, the same antibody clone in a different buffer formulation.

      Flow Cytometry analysis of Jurkat (human T cell leukemia T lymphocyte, Left) / HUVEC (human umbilical vein endothelial cell, Right) labeling EPCR/CD201 (red) with ab300526 at a 1/500 dilution. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Rabbit monoclonal IgG (ab172730) Isotype control (black). Unlabeled control - Cells without incubation with primary and secondary antibodies (blue).

      Gated on viable cells.

      Negative control: Jurkat (PMID: 11703343).

    • Immunoprecipitation - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)
      Immunoprecipitation - Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)

      This data was developed using ab300525, the same antibody clone in a different buffer formulation.

      EPCR/CD201 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate 10 ug with ab300525 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300525 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/1000 dilution.

      Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10 ug

      Lane 2: ab300525 IP in HUVEC whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300525 in HUVEC whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

    • Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)
      Anti-EPCR/CD201 antibody [EPR25647-5] - BSA and Azide free (AB300526)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300526 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300526 は論文での使用が確認できていません。

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