Anti-EPCR/CD201 抗体 [EPR25647-49] (BSA and Azide free) (ab300566)

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Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR25647-49] to EPCR/CD201 - BSA and Azide free
Suitable for: IHC-P, WB
Reacts with: Mouse, Human

Unconjugated

製品名

Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free)
EPCR/CD201 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR25647-49] to EPCR/CD201 - BSA and Azide free
由来種

Rabbit
アプリケーション

適用あり: IHC-P, WBmore details
適用なし: Flow Cyt,ICC/IF or IP
種交差性

交差種: Mouse, Human
非交差種: Rat
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HUVEC, Ea.Hy926, RKO, Jurkat, bEnd.3, B16-F10 whole cell lysates; HUVEC whole cell lysate treated with Protein Deglycosylation Mix II; human placenta, lung, skin tissue lysates. IHC-P: Human kidney, placenta, colon cancer FFPE tissue sections.
特記事項
ab300566 is the carrier-free version of ab300565.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.20
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR25647-49
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300566の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P		Use at an assay dependent concentration.
WB		Use at an assay dependent concentration.

特記事項
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

追加情報

Is unsuitable for Flow Cyt,ICC/IF or IP.

機能

Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
組織特異性

Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
翻訳後修飾

N-glycosylated.
A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form.
細胞内局在

Membrane.
Target information above from: UniProt accession Q9UNN8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 10544 Human
- Entrez Gene: 19124 Mouse
- Omim: 600646 Human
- SwissProt: Q9UNN8 Human
- SwissProt: Q64695 Mouse
- Unigene: 647450 Human
- Unigene: 3243 Mouse
別名
- Activated protein C receptor antibody
- APC receptor antibody
- APCR antibody
- bA42O4.2 antibody
- CCCA antibody
- CCD41 antibody
- CD201 antibody
- CD201 antigen antibody
- cell cycle, centrosome-associated protein antibody
- Cell cycle, centrosome-associated protein antibody
- centrocyclin antibody
- Endothelial cell protein C receptor antibody
- Endothelial protein C receptor antibody
- EPCR antibody
- EPCR_HUMAN antibody
- MGC23024 antibody
- PROCR antibody
- Protein C receptor, endothelial antibody
see all

Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lane 1 : bEnd.3 (mouse brain endothelioma), whole cell lysate
Lane 2 : B16-F10 (mouse skin melanoma mixture of spindle-shaped and epithelial-like), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 45 kDa why is the actual band size different from the predicted?

This data was developed using ab300565, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: B16-F10 (PMID: 19188668)

Exposure time: 3 minutes
Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lane 1 : Human placenta tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human skin tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 45 kDa why is the actual band size different from the predicted?

This data was developed using ab300565, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The band beneath the target band is supposed to be a soluble form. (PMID: 19806250)

Exposure time: Lane 1: 70 seconds Lane 2, 3: 3 minutes
Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lane 1 : Untreated HUVEC whole cell lysate
Lane 2 : HUVEC whole cell lysate treated with Protein Deglycosylation Mix II

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 23,45 kDa why is the actual band size different from the predicted?

This data was developed using ab300565, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

PROCR is a glycoprotein of approximately 45 kDa and detected as a 23kDa band after treated with Protein Deglycosylation MIX II.

Exposure time: 26 seconds
Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 2 : Ea.Hy926 (human somatic cell hybrid endothelial), whole cell lysate
Lane 3 : RKO (human colon epithelial cell), whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 45 kDa why is the actual band size different from the predicted?

This data was developed using 300565, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: Jurkat (PMID: 7929370)

The band beneath the target band is supposed to be a soluble form. (PMID: 19806250)

Exposure time: 15 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on human colon cancer (PMID: 12110501). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on human placenta (PMID:32003123). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

This data was developed using ab300565, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on endothelial cells of human kidney (PMID:26990852). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-EPCR/CD201 antibody [EPR25647-49] (BSA and Azide free) (AB300566)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

ab300566 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab300566 は論文での使用が確認できていません。

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

参考文献 (0)

レビューと Q&A