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リコンビナントRabMAb

Anti-EPCR/CD201 抗体 [EPR25647-49] (ab300565)

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Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
  • Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25647-49] to EPCR/CD201
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-EPCR/CD201 antibody [EPR25647-49]
    EPCR/CD201 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25647-49] to EPCR/CD201

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Mouse, Human
    非交差種: Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HUVEC, Ea.Hy926, RKO, Jurkat, bEnd.3, B16-F10 whole cell lysates; HUVEC whole cell lysate treated with Protein Deglycosylation Mix II; human placenta, lung, skin tissue lysates. IHC-P: Human kidney, placenta, colon cancer FFPE tissue sections.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300565の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000.
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.

  • 組織特異性

    Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.

  • 翻訳後修飾

    N-glycosylated.
    A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Activated protein C receptor antibody
      • APC receptor antibody
      • APCR antibody
      • bA42O4.2 antibody
      • CCCA antibody
      • CCD41 antibody
      • CD201 antibody
      • CD201 antigen antibody
      • cell cycle, centrosome-associated protein antibody
      • Cell cycle, centrosome-associated protein antibody
      • centrocyclin antibody
      • Endothelial cell protein C receptor antibody
      • Endothelial protein C receptor antibody
      • EPCR antibody
      • EPCR_HUMAN antibody
      • MGC23024 antibody
      • PROCR antibody
      • Protein C receptor, endothelial antibody
      see all

    画像

    • Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

      Lane 1 : bEnd.3 (mouse brain endothelioma), whole cell lysate
      Lane 2 : B16-F10 (mouse skin melanoma mixture of spindle-shaped and epithelial-like), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: B16-F10 (PMID: 19188668)

      Exposure time: 3 minutes

    • Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human lung tissue lysate
      Lane 3 : Human skin tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      The band beneath the target band is supposed to be a soluble form. (PMID: 19806250)

      Exposure time: Lane 1: 70 seconds Lane 2, 3: 3 minutes

    • Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

      Lane 1 : Untreated HUVEC whole cell lysate
      Lane 2 : HUVEC whole cell lysate treated with Protein Deglycosylation Mix II

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 23,45 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      PROCR is a glycoprotein of approximately 45 kDa and detected as a 23kDa band after treated with Protein Deglycosylation MIX II.

      Exposure time: 26 seconds

    • Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      Western blot - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      All lanes : Anti-EPCR/CD201 antibody [EPR25647-49] (ab300565) at 1/1000 dilution

      Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
      Lane 2 : Ea.Hy926 (human somatic cell hybrid endothelial), whole cell lysate
      Lane 3 : RKO (human colon epithelial cell), whole cell lysate
      Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: Jurkat (PMID: 7929370)

      The band beneath the target band is supposed to be a soluble form. (PMID: 19806250)

      Exposure time: 15 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

      Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on human colon cancer (PMID: 12110501). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

      Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on human placenta (PMID:32003123). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling EPCR/CD201 with ab300565 at 1/2000 dilution (0.524 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on endothelial cells of human kidney (PMID:26990852). The section was incubated with ab300565 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)
      Anti-EPCR/CD201 antibody [EPR25647-49] (AB300565)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300565 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300565 は論文での使用が確認できていません。

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