Anti-EpCAM 抗体 [VU-1D9] (ab187372)
![Flow Cytometry - Anti-EpCAM antibody [VU-1D9] (ab187372)](https://www.abcam.co.jp/ps/products/187/ab187372/Images/ab187372-479567-antiepcam-antibody-vu1d9-human-immunocytochemistry.jpg "Flow Cytometry - Anti-EpCAM antibody [VU-1D9] (ab187372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [VU-1D9] to EpCAM
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-EpCAM antibody [VU-1D9]
EpCAM 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [VU-1D9] to EpCAM -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human EpCAM.
Database link: P16422 -
ポジティブ･コントロール
- Flow Cyt: HT-29 cells. ICC/IF: HT-29, T47D and A431 cells.
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特記事項
This product was switched from a hybridoma to a recombinant production format on 26th October 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
VU-1D9 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187372の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/1000.
|
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| ICC/IF |
1/250.
|
| 特記事項 |
|---|
|
Flow Cyt
1/1000. |
|
ICC/IF
1/250. |
ターゲット情報
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機能
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. -
組織特異性
Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. -
関連疾患
Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM. -
配列類似性
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
翻訳後修飾
Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. -
細胞内局在
Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction. - Information by UniProt
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参照データベース
- Entrez Gene: 4072 Human
- Omim: 185535 Human
- SwissProt: P16422 Human
- Unigene: 542050 Human
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別名
- 17 1A antibody
- 323/A3 antibody
- Adenocarcinoma associated antigen antibody
see all
画像
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Flow Cytometry - Anti-EpCAM antibody [VU-1D9] (ab187372)
Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / HT-29 (Human colorectal adenocarcinoma epithelial cell, Right) labeling EpCAM with ab187372 at 1/1000 dilution, followed by secondary antibody ab150113 (Goat anti mouse IgG (Alexa Fluor® 488)) at 1/2000 dilution (Red). Compared with a Mouse monoclonal IgG isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody) (Blue). Gated on viable cells.
Negative control: Jurkat (PMID: 29352248)
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![Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [VU-1D9] (ab187372)](https://www.abcam.co.jp/ps/products/187/ab187372/Images/ab187372-479565-antiepcam-antibody-vu1d9-ht29-human-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [VU-1D9] (ab187372)ab187372 staining EpCAM in HT-29 cell line (top panel) and Jurkat negative cells (bottom panel). The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% TritonX-100. The cells were then incubated with ab187372 at 1/250 concentration and counterstained with ab179513 (Anti-beta Tubulin rabbit monoclonal antibody) at 1/200 dilution, followed by secondary antibody ab150113 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)) at 1/1000 dilution (shown in green) and counterstained with ab150080 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)) at 1/500 dilution (shown in red). Nuclear DNA was labelled in blue with DAPI.
Confocal image showing membranous staining in HT-29 cell line.
Negative control: Jurkat (PMID: 29352248） -
![Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [VU-1D9] (ab187372)](https://www.abcam.co.jp/ps/products/187/ab187372/Images/ab187372-4-anti-EpCAM-antibody-VU-1D9-immunocytochemistry-immunofluorescence-A431-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [VU-1D9] (ab187372)ab187372 staining EpCAM in wild-type A431 cells (top panel) and EpCAM knockout A431 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab187372 at 0.5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [VU-1D9] (ab187372)](https://www.abcam.co.jp/ps/products/187/ab187372/Images/ab187372-7-anti-EpCAM-antibody-VU-1D9-immunocytochemistry-immunofluorescence-T47D-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [VU-1D9] (ab187372)ab187372 staining EpCAM in T47D positive cells (top panel) and HeLa negative cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab187372 at 1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI. This antibody performed similarly using 100% methanol fixation. Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown. -
![Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [VU-1D9] (ab187372)](https://www.abcam.co.jp/ps/products/187/ab187372/Images/ab187372-6-anti-EpCAM-antibody-VU-1D9-immunocytochemistry-immunofluorescence-A431-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [VU-1D9] (ab187372)ab187372 staining EpCAM in wild-type A431 cells (top panel) and EpCAM knockout A431 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab187372 at 0.1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI. This antibody performed similarly using 100% methanol fixation. Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a single confocal section is shown.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab187372 は 5 報の論文で使用されています。
- Wang M et al. RhoJ facilitates angiogenesis in glioblastoma via JNK/VEGFR2 mediated activation of PAK and ERK signaling pathways. Int J Biol Sci 18:942-955 (2022). PubMed: 35173528
- Dai Y et al. A Multivariate Diagnostic Model Based on Urinary EpCAM-CD9-Positive Extracellular Vesicles for Prostate Cancer Diagnosis. Front Oncol 11:777684 (2021). PubMed: 34900726
- van den Brand D et al. EpCAM-Binding DARPins for Targeted Photodynamic Therapy of Ovarian Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32630661
- Parker SG et al. A photoelectrochemical platform for the capture and release of rare single cells. Nat Commun 9:2288 (2018). PubMed: 29895867
- Bohmer N et al. Removal of Cells from Body Fluids by Magnetic Separation in Batch and Continuous Mode: Influence of Bead Size, Concentration, and Contact Time. ACS Appl Mater Interfaces 9:29571-29579 (2017). PubMed: 28805365