AB46714

Anti-EpCAM 抗体 [HEA125], prediluted

Name: Anti-EpCAM 抗体 [HEA125], prediluted (ab46714) | Abcam
Brand: Abcam Limited
SKU: AB46714
Price: 71000 JPY
Availability: InStock

Anti-EpCAM antibody [HEA125], prediluted

Be the first to review this product! Submit a review

(4 Publications)

Mouse Monoclonal EPCAM antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing EPCAM protein.

別名を表示する

CD326, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1, EPCAM, Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, EGP, EGP314, hEGP314

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (AB46714)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [HEA125], prediluted (AB46714)

ICC/IF image of ab46714 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA, 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab46714 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

HEA125

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

Cell preparation containing EPCAM protein. The exact immunogen used to generate this antibody is proprietary information.

P16422

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

製品の詳細

ab46714 reacts intensely with all carcinomas and metastased derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate. Stratified squamous epithelia usually stain less intensely than adenocarcinoma. The antibody is suitable for differentiation between carcinoma and non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained, as do sarcoma, lymphoma, melanoma and neurogenic tumours.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: BSA, PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

EpCAM also known as epithelial cell adhesion molecule is a transmembrane glycoprotein with a molecular weight of around 40 kDa. Alternative names include CD326 and TACSTD1. This protein is expressed on the surface of most epithelial cells and plays a role in cell adhesion. It is involved in maintaining the integrity of epithelial tissues by mediating homotypic cell-cell interactions which are important for the structure and function of tissues.

Biological function summary

EpCAM facilitates signaling pathways that impact cellular processes like proliferation and differentiation. It is not a part of a larger protein complex but interacts directly with other cellular machinery to transmit intracellular signals. EpCAM influences stem cell behavior and is important in tissue homeostasis assisting in both normal regenerative processes and wound healing.

Pathways

EpCAM participates in regulatory mechanisms involving the Wnt/β-catenin signaling pathway which affects gene expression and cell fate decisions. Through its action EpCAM modulates components like E-cadherin which is important for cell adhesion and the maintenance of the epithelial layer. It indirectly influences pathways relating to MYC a transcription factor that regulates cell cycle progression and apoptosis.

EpCAM is associated with various types of cancer including colorectal and breast cancer. Its overexpression often correlates with aggressive cancer phenotypes and poor prognosis. Besides cancers EpCAM mutations can lead to congenital tufting enteropathy a rare condition affecting intestinal epithelial structure and function. Aberrant EpCAM expression affects β-catenin levels impacting cellular pathways linked to tumor progression and epithelial integrity.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.

See full target information EPCAM

style

left-column

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

ImmunoHorizons 8:500-510 PubMed39018546

2024

Comprehensive Flow Cytometric, Immunohistologic, and Molecular Assessment of Thymus Function in Rhesus Macaques.

Applications

Unspecified application

Species

Unspecified reactive species

Laura P Hale,Andrew N Macintyre,Dawn E Bowles,Jean Kwun,Jie Li,Barbara Theriot,Joseph W Turek

Bio-protocol 8:e3124 PubMed34532562

2018

Monitoring Natural Killer Cell Function in Human Ovarian Cancer Cells of Ascitic Fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Vikash Kumar,Asok Mukhopadhyay

Nature communications 9:894 PubMed29497050

2018

Whole-exome sequencing reveals the origin and evolution of hepato-cholangiocarcinoma.

Applications

IHC-P

Species

Human

Anqiang Wang,Liangcai Wu,Jianzhen Lin,Longzhe Han,Jin Bian,Yan Wu,Simon C Robson,Lai Xue,Yunxia Ge,Xinting Sang,Wenze Wang,Haitao Zhao

World journal of hepatology 5:425-32 PubMed24023981

2013

Participation of peribiliary glands in biliary tract pathophysiologies.

Applications

Unspecified application

Species

Unspecified reactive species

Saya Igarashi,Yasunori Sato,Xiang Shan Ren,Kenichi Harada,Motoko Sasaki,Yasuni Nakanuma

View all publications

style

left-column

style

left-column

style

right-column

代替製品

Primary Antibodies

AB237387

PE Anti-EpCAM antibody [EPR20533-63]

RabMAb|Recombinant

Flow Cytometry - PE Anti-EpCAM antibody [EPR20533-63] (AB237387)

Primary Antibodies

AB213500

Anti-EpCAM antibody [EPR20532-222]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20532-222] (AB213500)

Primary Antibodies

AB124825

Anti-EpCAM antibody [EPR677(2)]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR677(2)] (AB124825)

Primary Antibodies

AB225894

Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free (AB225894)

Primary Antibodies

AB221552

Anti-EpCAM antibody [EPR20533-63]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-63] (AB221552)

Primary Antibodies

AB213501

Anti-EpCAM antibody [EPR20533-266]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [EPR20533-266] (AB213501)

Primary Antibodies

AB32392

Anti-EpCAM antibody [E144]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-EpCAM antibody [E144] (AB32392)

Primary Antibodies

AB223582

Anti-EpCAM antibody [EPR20532-225]

Immunocytochemistry/ Immunofluorescence - Anti-EpCAM antibody [EPR20532-225] (AB223582)

組合せ製品

Assay Kits

AB264632

Human EpCAM ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human EpCAM ELISA Kit (AB264632)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

Primary Antibodies

AB52625

Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (AB52625)

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com