Anti-EPB41 抗体 (ab185704)
Key features and details
- Rabbit polyclonal to EPB41
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EPB41 antibody -
製品の詳細
Rabbit polyclonal to EPB41 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human EPB41 aa 210-500.
Database link: P11171 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185704の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 97 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 97 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. -
関連疾患
Defects in EPB41 are the cause of elliptocytosis type 1 (EL1) [MIM:611804]. EL1 is a Rhesus-linked form of hereditary elliptocytosis, a genetically heterogeneous, autosomal dominant, hematologic disorder. It is characterized by variable hemolytic anemia and elliptical or oval red cell shape.
Defects in EPB41 are a cause of hereditary pyropoikilocytosis (HPP) [MIM:266140]. HPP is an autosomal recessive hematologic disorder characterized by hemolytic anemia, microspherocytosis, poikilocytosis, and an unusual thermal sensitivity of red cells. -
配列類似性
Contains 1 FERM domain. -
翻訳後修飾
Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.
Phosphorylation on Tyr-660 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.
O-glycosylated; contains N-acetylglucosamine side chains in the C-terminal domain. -
細胞内局在
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2035 Human
- Entrez Gene: 269587 Mouse
- Omim: 130500 Human
- SwissProt: P11171 Human
- SwissProt: P48193 Mouse
- Unigene: 175437 Human
- Unigene: 708933 Human
- Unigene: 712722 Human
see all -
別名
- 4.1R antibody
- 41_HUMAN antibody
- Band 4.1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-EPB41 antibody (ab185704)Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185704. Blue DAPI for nuclear staining.
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Western blot - Anti-EPB41 antibody (ab185704)All lanes : Anti-EPB41 antibody (ab185704) at 1/500 dilution
Lane 1 : 293T cell lysate
Lane 2 : mouse brain lysate
Lane 3 : mouse liver lysate
Predicted band size: 97 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab185704 は 1 報の論文で使用されています。
- Dekel E et al. 20S proteasomes secreted by the malaria parasite promote its growth. Nat Commun 12:1172 (2021). PubMed: 33608523