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リコンビナントRabMAb

Anti-ENSA 抗体 [EPR8008(2)] (ab180513)

Submit a review Submit a question References (1)
Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513)
  • Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513)
  • Flow Cytometry (Intracellular) - Anti-ENSA antibody [EPR8008(2)] (ab180513)
  • Anti-ENSA antibody [EPR8008(2)] (ab180513)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8008(2)] to ENSA
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ENSA antibody [EPR8008(2)]
    ENSA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR8008(2)] to ENSA

  • 由来種

    Rabbit

  • 特異性

    ENSA (O43768) and ARPP-19 (P56211)

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562 cells, HeLa whole cell lysate (ab150035), Fetal brain, Rat and Mouse heart tissue lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180513の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
特記事項
Flow Cyt (Intra)
1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.

ターゲット情報

  • 機能

    Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

  • 組織特異性

    Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas.

  • 配列類似性

    Belongs to the endosulfine family.

  • 翻訳後修飾

    Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Alpha endosulfine antibody
    • Alpha-endosulfine antibody
    • ARPP 19e antibody
    • ARPP-19e antibody
    • Endosulfine alpha antibody
    • ENSA antibody
    • ENSA_HUMAN antibody
    see all

画像

  • Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513)
    Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513)
    All lanes : Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/10000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Mouse heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution

    Predicted band size: 13 kDa



    Blocking/dilutin buffer 5% NFDM/TBST

  • Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513)
    Western blot - Anti-ENSA antibody [EPR8008(2)] (ab180513)
    Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/2000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody at 1/1000 dilution

    Predicted band size: 13 kDa



    Blocking/diuting buffer buffer 5% NFDM/TBST

  • Flow Cytometry (Intracellular) - Anti-ENSA antibody [EPR8008(2)] (ab180513)
    Flow Cytometry (Intracellular) - Anti-ENSA antibody [EPR8008(2)] (ab180513)

    Intracellular flow cytometric analysis of K562 cells labeling ENSAwith ab180513 at 1/70 dilution. Goat anti rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Cells were fixed in 2% paraformaldehyde. 

     

     

  • Anti-ENSA antibody [EPR8008(2)] (ab180513)
    Anti-ENSA antibody [EPR8008(2)] (ab180513)

参考文献 (1)

ab180513 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab180513 は 1 報の論文で使用されています。

  • Gouttia OG  et al. The MASTL-ENSA-PP2A/B55 axis modulates cisplatin resistance in oral squamous cell carcinoma. Front Cell Dev Biol 10:904719 (2022). PubMed: 36247015

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