Anti-ENSA 抗体 [EPR8008(2)] (ab180513)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8008(2)] to ENSA
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ENSA antibody [EPR8008(2)]
ENSA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8008(2)] to ENSA -
由来種
Rabbit -
特異性
ENSA (O43768) and ARPP-19 (P56211) -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- K562 cells, HeLa whole cell lysate (ab150035), Fetal brain, Rat and Mouse heart tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8008(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180513の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
|
特記事項 |
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Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa. |
ターゲット情報
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機能
Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents. -
組織特異性
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. -
配列類似性
Belongs to the endosulfine family. -
翻訳後修飾
Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2029 Human
- Entrez Gene: 56205 Mouse
- Entrez Gene: 60334 Rat
- Omim: 603061 Human
- SwissProt: O43768 Human
- SwissProt: P60840 Mouse
- SwissProt: P60841 Rat
- Unigene: 632456 Human
see all -
別名
- Alpha endosulfine antibody
- Alpha-endosulfine antibody
- ARPP 19e antibody
see all
画像
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All lanes : Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/10000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
Predicted band size: 13 kDaBlocking/dilutin buffer 5% NFDM/TBST
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Anti-ENSA antibody [EPR8008(2)] (ab180513) at 1/2000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody at 1/1000 dilution
Predicted band size: 13 kDaBlocking/diuting buffer buffer 5% NFDM/TBST
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Intracellular flow cytometric analysis of K562 cells labeling ENSAwith ab180513 at 1/70 dilution. Goat anti rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Cells were fixed in 2% paraformaldehyde.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab180513 は 1 報の論文で使用されています。
- Gouttia OG et al. The MASTL-ENSA-PP2A/B55 axis modulates cisplatin resistance in oral squamous cell carcinoma. Front Cell Dev Biol 10:904719 (2022). PubMed: 36247015