Anti-eNOS (phospho S632) 抗体 [M232] (ab76199)
![Western blot - Anti-eNOS (phospho S632) antibody [M232] (ab76199)](https://www.abcam.co.jp/ps/products/76/ab76199/Images/ab76199-91558-anti-enos-phospho-s632-antibody-m232-western-blot.gif "Western blot - Anti-eNOS (phospho S632) antibody [M232] (ab76199)"){kind=small}
Key features and details
- Mouse monoclonal [M232] to eNOS (phospho S632)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-eNOS (phospho S632) antibody [M232]
eNOS 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M232] to eNOS (phospho S632) -
由来種
Mouse -
特異性
ab76199 is not expected to react with other NOS family members due to low homology. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
-
免疫原
Synthetic peptide corresponding to Mouse eNOS (phospho S632).
Database link: P70313 -
ポジティブ･コントロール
- Human umbilical vein endothelial cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
M232 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76199の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa).
|
| 特記事項 |
|---|
|
WB
1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa). |
ターゲット情報
-
機能
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
組織特異性
Platelets, placenta, liver and kidney. -
関連疾患
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
翻訳後修飾
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
細胞内局在
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
-
参照データベース
- Entrez Gene: 4846 Human
- Entrez Gene: 24600 Rat
- Omim: 163729 Human
- SwissProt: P29474 Human
- SwissProt: Q62600 Rat
- Unigene: 647092 Human
- Unigene: 44265 Rat
-
別名
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
see all
画像
-
Western blot - Anti-eNOS (phospho S632) antibody [M232] (ab76199)All lanes : Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilution
Lane 1 : human umbilical vein endothelial
cells, untreated
Lane 2 : human umbilical vein endothelial
cells, treated with lambda phosphatase
Predicted band size: 133 kDa
Observed band size: 120,140 kDa why is the actual band size different from the predicted?
The 120 kDa band may be a truncated form of eNOS.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (15)
ab76199 は 15 報の論文で使用されています。
- Guan Y & Wang X Salvianic Acid A Regulates High-Glucose-Treated Endothelial Progenitor Cell Dysfunction via the AKT/Endothelial Nitric Oxide Synthase (eNOS) Pathway. Med Sci Monit 27:e928153 (2021). PubMed: 33770068
- Cheng X et al. Inhibition of LOXL1-AS1 alleviates oxidative low-density lipoprotein induced angiogenesis via downregulation of miR-590-5p mediated KLF6/VEGF signaling pathway. Cell Cycle 20:1663-1680 (2021). PubMed: 34334119
- Ye P et al. Endothelial Agrin Is Dispensable for Normal and Tumor Angiogenesis. Front Cardiovasc Med 8:810477 (2021). PubMed: 35174224
- Xu J et al. Knockdown of Long Noncoding RNA (lncRNA) AK094457 Relieved Angiotensin II Induced Vascular Endothelial Cell Injury. Med Sci Monit 26:e919854 (2020). PubMed: 32027625
- Xu H et al. GATA4 protects against hyperglycemia-induced endothelial dysfunction by regulating NOX4 transcription. Mol Med Rep 17:1485-1492 (2018). PubMed: 29138836