Anti-eNOS (phospho S1177) 抗体 (ab184154)
Key features and details
- Rabbit polyclonal to eNOS (phospho S1177)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-eNOS (phospho S1177) antibody
eNOS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to eNOS (phospho S1177) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Guinea pig, Pig
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免疫原
Synthetic peptide within Human eNOS (phospho S1177). The exact sequence is proprietary.
Database link: P29474 -
ポジティブ･コントロール
- THP1 whole cell lysate (untreated and treated with 100 nM PMA treatment for 6, 24 or 48 hr), Jurkat, Raji and NCI-H929 whole cell lysate; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184154の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 133 kDa.
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| ICC/IF | (1) |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 133 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
組織特異性
Platelets, placenta, liver and kidney. -
関連疾患
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
配列類似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
翻訳後修飾
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
細胞内局在
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
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参照データベース
- Entrez Gene: 100135577 Guinea pig
- Entrez Gene: 4846 Human
- Entrez Gene: 397557 Pig
- Omim: 163729 Human
- SwissProt: P97270 Guinea pig
- SwissProt: P29474 Human
- SwissProt: Q28969 Pig
- Unigene: 647092 Human
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別名
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
see all
画像
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Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)All lanes : Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lane 1 : Untreated THP-1 whole cell extracts
Lane 2 : THP-1 whole cell extracts treated with 100nM PMA, 6 hr
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 133 kDa5% SDS-PAGE
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Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)Anti-eNOS (phospho S1177) antibody (ab184154) at 1/1000 dilution + HUVEC whole cell extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 133 kDa -
Western blot - Anti-eNOS (phospho S1177) antibody (ab184154)All lanes : Anti-eNOS (phospho S1177) antibody (ab184154) at 1/500 dilution
Lane 1 : Jurkat whole cell extract
Lane 2 : Raji whole cell extract
Lane 3 : NCI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 133 kDa5% SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (46)
ab184154 は 46 報の論文で使用されています。
- De Logu F et al. Schwann cell endosome CGRP signals elicit periorbital mechanical allodynia in mice. Nat Commun 13:646 (2022). PubMed: 35115501
- Chianese U et al. Histone lysine demethylase inhibition reprograms prostate cancer metabolism and mechanics. Mol Metab 64:101561 (2022). PubMed: 35944897
- Smith JA et al. Young Women Are Protected Against Vascular Insulin Resistance Induced by Adoption of an Obesogenic Lifestyle. Endocrinology 163:N/A (2022). PubMed: 35974454
- Ghiarone T et al. ADAM17 cleaves the insulin receptor ectodomain on endothelial cells and causes vascular insulin resistance. Am J Physiol Heart Circ Physiol 323:H688-H701 (2022). PubMed: 36018759
- Ouyang R et al. FGF21 attenuates high uric acid‑induced endoplasmic reticulum stress, inflammation and vascular endothelial cell dysfunction by activating Sirt1. Mol Med Rep 25:N/A (2022). PubMed: 34850960