AB157474

Anti-ENO1 + ENO3 抗体 [EPR11366(B)]

Anti-ENO1 + ENO3 antibody [EPR11366(B)]

RabMAb
Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal ENO3 antibody. Suitable for WB and reacts with Human, Recombinant fragment samples. Cited in 2 publications.

別名を表示する

Beta-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 3, Muscle-specific enolase, Skeletal muscle enolase, MSE, ENO3

3 Images

Unknown

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

All lanes:

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Lane 4:

Fetal heart lysate at 10 µg

Lane 5:

Fetal muscle lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

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Unknown

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Note : primary antibody diluted with 1% BSA.

All lanes:

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from fetal muscle lysate

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG

Predicted band size: 47 kDa

false

Lab

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Mouse anti-6x HisTag was used as a loading control.

All lanes:

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (ab157474) at 1/2000 dilution

Lane 1:

Human ENO3 protein (His Tag) at 0.2 µg

Lane 2:

Human ENO2 protein (His Tag) at 0.2 µg

Lane 3:

Human ENO1 protein (His Tag) at 0.2 µg

Predicted band size: 47 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR11366(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The ENO3 protein also known as beta-enolase mechanically functions as an enzyme involved in glycolysis catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This protein has a molecular mass of approximately 47 kDa. It is specifically expressed in skeletal muscle tissue where it plays a critical role in energy metabolism. Beta-enolase is an isoform of enolase that is distinct from the alpha (ENO1) and gamma (ENO2) isoforms which have different tissue specificities.

Biological function summary

Beta-enolase supports cellular energy production in muscles. It does not typically form part of a protein complex but acts independently in the glycolytic pathway. Its function in glycolysis is important for providing ATP to muscle cells especially during periods of high energy demand such as exercise. The efficient conversion of metabolic substrates in this pathway is essential for muscle function and maintenance.

Pathways

Beta-enolase operates within the glycolytic pathway and plays an important role in energy production. This pathway is fundamental for breaking down glucose to provide ATP the energy currency of the cell. Beta-enolase works closely with other key glycolytic enzymes such as hexokinase and phosphofructokinase. Its role is indispensable in maintaining the flow of carbon through glycolysis ensuring robust energy supply for muscle contraction.

Beta-enolase is associated with diseases like glycogen storage disease type XIII and muscle-related disorders. Mutations in ENO3 gene can lead to reduced energetic efficiency and impact muscular health. Of particular note is the connection with related glycolytic proteins which may exhibit compensatory or contributing roles in these disorders. This highlights the importance of beta-enolase and its interactions in maintaining muscle physiology and energy homeostasis.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. Appears to have a function in striated muscle development and regeneration.

See full target information ENO3

追加のターゲット

ENO1

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Plant journal : for cell and molecular biology 120:123-138 PubMed39145515

2024

The eukaryotic translation initiation factor eIF4E regulates flowering and circadian rhythm in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Huanhuan Jin,Yadi Chen,Yonghong Jiang,Lianfeng Gu,Guifang Lin,Chentao Lin,Qin Wang

Mediators of inflammation 2023:8052579 PubMed37448887

2023

LINC00174 Promotes Colon Cancer Progression by Regulating Inflammation and Glycolysis by Targeting the MicroRNA-2467-3p/Enolase 3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Xu,Jiawei Lin,Rong Chen,Junjie Xie,Enquan Yuan,Fajie Cen,Fanbiao Kong

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENO1 + ENO3 抗体 [EPR11366(B)]

Anti-ENO1 + ENO3 antibody [EPR11366(B)]

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存温度

長期保存温度

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (2)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Abcam product promise