Anti-Endothelium 抗体 [PAL-E] (ab8086)
Key features and details
- Mouse monoclonal [PAL-E] to Endothelium
- Suitable for: IHC-Fr
- Reacts with: Rabbit, Goat, Dog, Human, Pig
- Isotype: IgG2a
製品の概要
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製品名
Anti-Endothelium antibody [PAL-E]
Endothelium 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PAL-E] to Endothelium -
由来種
Mouse -
特異性
Reactivity with: Lymphatic endothelium :not detectable. Sinus histiocytes :not detectable. Megakaryocytes :not detectable. -
アプリケーション
適用あり: IHC-Frmore details
適用なし: WB -
種交差性
交差種: Rabbit, Goat, Dog, Human, Pig
非交差種: Mouse, Rat, Chicken -
免疫原
Tissue, cells or virus corresponding to Human Endothelium.
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ポジティブ・コントロール
- IHC-Fr: human spleen tissue
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特記事項
The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.3
Preservative: 0.07% Sodium azide -
Concentration information loading...
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精製度
Tissue culture supernatant -
一次抗体 備考
The antibody only stains human and various animal (goat, rabbit, pig, dog) blood vessels with the exception of arteries. -
ポリ/モノ
モノクローナル -
クローン名
PAL-E -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8086の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-Fr | (1) |
1/100.
Fix with acetone. |
特記事項 |
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IHC-Fr
1/100. Fix with acetone. |
ターゲット情報
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関連性
The endothelium is located at the interface between the blood and the vessel wall. The cells are in close contact and form a slick layer that prevents blood cell interaction with the vessel wall as blood moves through the vessel lumen. The endothelium consists of simple squamous epithelium that lines the lumen of all blood vessels. It plays a critical role in the mechanics of blood flow, the regulation of coagulation, leukocyte adhesion, and vascular smooth muscle cell growth, and also serves as a barrier to the transvascular diffusion of liquids and solutes. For years the endothelium was thought of as an inert single layer of cells that passively allow the passage of water and other small molecules across the vessel wall. However, this dynamic tissue performs many other active functions, such as the secretion and modification of vasoactive substances and the contraction and relaxation of vascular smooth muscle.
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab8086 は 12 報の論文で使用されています。
- Wilkinson AL et al. The senescent secretome drives PLVAP expression in cultured human hepatic endothelial cells to promote monocyte transmigration. iScience 26:107966 (2023). PubMed: 37810232
- Elkadri A et al. Mutations in Plasmalemma Vesicle Associated Protein Result in Sieving Protein-Losing Enteropathy Characterized by Hypoproteinemia, Hypoalbuminemia, and Hypertriglyceridemia. Cell Mol Gastroenterol Hepatol 1:381-394.e7 (2015). PubMed: 26207260
- Keuschnigg J et al. Plasticity of blood- and lymphatic endothelial cells and marker identification. PLoS One 8:e74293 (2013). PubMed: 24058540
- Park SS et al. Long-term effects of intravitreal injection of GMP-grade bone-marrow-derived CD34+ cells in NOD-SCID mice with acute ischemia-reperfusion injury. Invest Ophthalmol Vis Sci 53:986-94 (2012). PubMed: 22247454
- Moussai D et al. The human cutaneous squamous cell carcinoma microenvironment is characterized by increased lymphatic density and enhanced expression of macrophage-derived VEGF-C. J Invest Dermatol 131:229-36 (2011). PubMed: 20827282