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AB8087

Anti-Endothelium 抗体 [EN4]

Anti-Endothelium antibody [EN4]

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(6 Publications)

Mouse Monoclonal Endothelium antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Guinea pig, Human, Cat samples. Cited in 6 publications.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

EN4

アイソタイプ

IgG1

キャリアフリー

No

交差種

Guinea pig, Cat, Human

アプリケーション

IHC-Fr, WB, IHC-P

applications

特異性

This antibody stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen (ab8086) which is lost. It stains strongly murine fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.

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Reactivity data

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製品の詳細

The antibody is useful for immunohistological staining of endothelia. Lymphatic endothelium is stained (unlike the PAL-E antibody). Kaposi's sarcoma and other vascular tumors of endothelial cell origin are also stained.
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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The endothelium a monolayer of cells lining blood vessels plays an important role in vascular function and maintenance. Endothelial cells have a mass of approximately 35 kDa and are found in arteries veins and capillaries including the heart and lymphatic vessels. These cells form a selective barrier between the bloodstream and surrounding tissues regulating the exchange of fluids solutes and macromolecules. The endothelium also modulates blood flow and pressure through interaction with vascular smooth muscle cells.
Biological function summary

The endothelium contributes to several critical processes including vascular homeostasis coagulation and inflammation regulation. It operates as part of the vascular system interacting with components like platelets and leukocytes to mediate immune responses. The endothelium produces signaling molecules such as nitric oxide (NO) which is essential for vasodilation. This layer also responds to mechanical stimuli from blood flow adapting its structure and function accordingly.

Pathways

The endothelium engages in the nitric oxide and endothelin pathways which balance vasodilation and vasoconstriction. In the nitric oxide pathway the enzyme endothelial nitric oxide synthase (eNOS) becomes activated producing NO that signals smooth muscle relaxation. Within the endothelin pathway endothelin-1 interacts with vascular smooth muscle cells causing vasoconstriction. Both pathways are integral to maintaining vascular tone and regulating blood pressure.

The endothelium's dysfunction is associated with conditions such as atherosclerosis and hypertension. In atherosclerosis the impaired endothelial function leads to plaque formation and arterial stiffness. Hypertension involves chronic constriction and reduced NO bioavailability affecting endothelium-mediated vasodilation. Related proteins include low-density lipoprotein (LDL) for atherosclerosis and angiotensin-converting enzyme (ACE) in hypertension both impacting endothelial health and function.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information Endothelium
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Angiogenesis 15:151-63 PubMed22249946

2012

CD34 marks angiogenic tip cells in human vascular endothelial cell cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Martin J Siemerink,Ingeborg Klaassen,Ilse M C Vogels,Arjan W Griffioen,Cornelis J F Van Noorden,Reinier O Schlingemann

Molecular neurodegeneration 6:39 PubMed21645391

2011

Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice.

Applications

WB

Species

Mouse

Cristian A Lasagna-Reeves,Diana L Castillo-Carranza,Urmi Sengupta,Audra L Clos,George R Jackson,Rakez Kayed

Lung cancer (Amsterdam, Netherlands) 50:177-88 PubMed16043261

2005

Oxytocin receptor pattern of expression in primary lung cancer and in normal human lung.

Applications

IHC-Fr

Species

Human

Christel Péqueux,Christophe Breton,Marie-Thérèse Hagelstein,Vincent Geenen,Jean-Jacques Legros

Clinical and experimental immunology 96:170-6 PubMed7512006

1994

Characterization of EN4 monoclonal antibody: a reagent with CD31 specificity.

Applications

Unspecified application

Species

Unspecified reactive species

V L Burgio,S Zupo,S Roncella,M Zocchi,L P Ruco,C D Baroni

Journal of immunological methods 91:45-52 PubMed3014005

1986

The detection of endothelial cell antigens in cutaneous tissue using methacarn and periodate lysine paraformaldehyde fixation.

Applications

Unspecified application

Species

Unspecified reactive species

C A Holden,J Spaull,R Williams,C J Spry,R R Jones,E W Jones

Journal of clinical pathology 39:742-9 PubMed3090109

1986

Histogenesis of Kaposi's sarcoma in patients with and without acquired immune deficiency syndrome (AIDS).

Applications

Unspecified application

Species

Unspecified reactive species

R R Jones,J Spaull,C Spry,E W Jones
View all publications
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代替製品

Primary Antibodies

AB8086

Anti-Endothelium antibody [PAL-E]

Immunohistochemistry (Frozen sections) - Anti-Endothelium antibody [PAL-E] (AB8086)

  • 3
  • 2

組合せ製品

Primary Antibodies

AB151712

Anti-VAMP1 antibody [EPR7325(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (AB151712)

  • 5
  • 1
Primary Antibodies

AB8086

Anti-Endothelium antibody [PAL-E]

Immunohistochemistry (Frozen sections) - Anti-Endothelium antibody [PAL-E] (AB8086)

  • 3
  • 2
Primary Antibodies

AB9774

Anti-Endothelial Cell antibody [RECA-1]

BOND RX™ Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelial Cell antibody [RECA-1] (AB9774)

  • 5
  • 19
Primary Antibodies

AB28364

Anti-CD31 antibody

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody (AB28364)

  • 4
  • 160

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