Anti-Endothelin B Receptor/ET-B 抗体 (ab117529)
Key features and details
- Rabbit polyclonal to Endothelin B Receptor/ET-B
- Suitable for: IHC-Fr, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Endothelin B Receptor/ET-B antibody -
製品の詳細
Rabbit polyclonal to Endothelin B Receptor/ET-B -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Fr, ICC/IF, IHC-Pmore details
適用なし: WB -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Goat, Horse, Chicken, Cow, Dog, Pig, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Mouse Endothelin B Receptor/ET-B aa 400-500 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P48302 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117529の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
1/500 - 1/2000.
|
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| ICC/IF | (2) |
1/5000.
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| IHC-P | (1) |
1/2000.
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| 特記事項 |
|---|
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IHC-Fr
1/500 - 1/2000. |
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ICC/IF
1/5000. |
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IHC-P
1/2000. |
ターゲット情報
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機能
Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
組織特異性
Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. -
関連疾患
Defects in EDNRB are a cause of Waardenburg syndrome type 4A (WS4A) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4A is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). HSCR2 is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.
Defects in EDNRB are the cause of ABCD syndrome (ABCDS) [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRB sub-subfamily. -
翻訳後修飾
Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 408082 Chicken
- Entrez Gene: 281750 Cow
- Entrez Gene: 100033875 Horse
- Entrez Gene: 1910 Human
- Entrez Gene: 13618 Mouse
- Entrez Gene: 100009477 Rabbit
- Omim: 131244 Human
- SwissProt: P28088 Cow
see all -
別名
- ABCDS antibody
- Ednra antibody
- EDNRB antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529.
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Immunohistochemistry (Frozen sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)Immunohistochemistry (Frozen sections) analysis of mouse skin tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/800 dilution. Tissues were fixed for 10 minutes, blocked in 3% fish gelatin for 10-20 minutes, and probed with an Isotype Control Antibody (left panel) (1/800 dilution), and Anti-Endothelin B Receptor antibody (ab117529) (right panel) overnight at 4ºC. Detection was performed using a PE-conjugated anti-rabbit IgG secondary antibody at a dilution of 1/800 (red). Nuclei were stained with DAPI and tissues were visualized by fluorescence microscopy.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Endothelin B Receptor/ET-B antibody (ab117529)This image is courtesy of an anonymous abreview.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling Endothelin B Receptor/ET-B with ab117529 at 1/1500 dilution. Tissue was fixed with formaldehyde; heat mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C. The tissue was incubated with Anti-Endothelin B Receptor antibody (ab117529) for 12 hours in PBS at 20°C. A biotin conjugated goat anti-rabbit secondary antibody at 1/200 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Endothelin B Receptor/ET-B antibody (ab117529)ab117529, at 1/5000 dilution, staining Endothelin B Receptor/ET-B in ETB-transfected HEK293 cells by Immunocytochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab117529 は 36 報の論文で使用されています。
- Shi CJ et al. TGFβR-1/ALK5 inhibitor RepSox induces enteric glia-to-neuron transition and influences gastrointestinal mobility in adult mice. Acta Pharmacol Sin 44:92-104 (2023). PubMed: 35794374
- Bonnamour G et al. NR2F1 regulates a Schwann cell precursor-vs-melanocyte cell fate switch in a mouse model of Waardenburg syndrome type IV. Pigment Cell Melanoma Res 35:506-516 (2022). PubMed: 35816394
- Weigert A et al. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system. Nat Commun 13:6078 (2022). PubMed: 36241617
- Zhang Y et al. Endothelin-1, over-expressed in SOD1G93A mice, aggravates injury of NSC34-hSOD1G93A cells through complicated molecular mechanism revealed by quantitative proteomics analysis. Front Cell Neurosci 16:1069617 (2022). PubMed: 36531135
- van de Wouw J et al. Impaired pulmonary vasomotor control in exercising swine with multiple comorbidities. Basic Res Cardiol 116:51 (2021). PubMed: 34510273