Anti-Endothelin A Receptor/ET-A 抗体 (ab85163)
Key features and details
- Rabbit polyclonal to Endothelin A Receptor/ET-A
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Endothelin A Receptor/ET-A antibody
Endothelin A Receptor/ET-A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Endothelin A Receptor/ET-A -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Rabbit, Dog, Human
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免疫原
Synthetic peptide corresponding to Rat Endothelin A Receptor/ET-A aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93498) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab85163の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 48 kDa). |
ターゲット情報
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機能
Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. -
組織特異性
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 450187 Dog
- Entrez Gene: 1909 Human
- Entrez Gene: 13617 Mouse
- Entrez Gene: 100126073 Rabbit
- Entrez Gene: 24326 Rat
- GenBank: NM_001957 Human
- GenBank: NM_174308 Human
- GenBank: NM_012550 Rat
see all -
別名
- Ednra antibody
- EDNRA_HUMAN antibody
- Endothelin 1 receptor precursor antibody
see all
画像
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Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab85163)All lanes : Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
The band observed at 46 kDa could potentially be a cleaved form of Rat Endothelin-1 receptor precursor, as the target protein contains a 20 amino acid signal peptide, which when removed gives an expected molecular weight of 46 kDa. -
Western blot - Anti-Endothelin A Receptor/ET-A antibody (ab85163)This image is courtesy of an Abreview submitted by Nico JentschAll lanes : Anti-Endothelin A Receptor/ET-A antibody (ab85163) at 1/250 dilution (in 5% BSA + TBS + 0.1% Tween20 for 12 hours at 4°C)
All lanes : Whole tissue lysate of rat lung
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 30 seconds
Blocking step: 5% Milk for 1 hour at 23°C
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab85163 は 20 報の論文で使用されています。
- Lee YJ et al. The EDN1/EDNRA/β‑arrestin axis promotes colorectal cancer progression by regulating STAT3 phosphorylation. Int J Oncol 62:N/A (2023). PubMed: 36453252
- Karakaya E et al. Porphyromonas gingivalis infection upregulates the endothelin (ET) system in brain microvascular endothelial cells. Can J Physiol Pharmacol 100:679-688 (2022). PubMed: 35442801
- Li C et al. Rho kinase inhibition ameliorates vascular remodeling and blood pressure elevations in a rat model of apatinib-induced hypertension. J Hypertens 40:675-684 (2022). PubMed: 34862331
- Qin C et al. The Involvement of Endothelin Pathway in Chronic Psychological Stress-Induced Bladder Hyperalgesia Through Capsaicin-Sensitive C-Fiber Afferents. J Inflamm Res 15:1209-1226 (2022). PubMed: 35228812
- Wu CZ et al. NOX4/Src regulates ANP secretion through activating ERK1/2 and Akt/GATA4 signaling in beating rat hypoxic atria. Korean J Physiol Pharmacol 25:159-166 (2021). PubMed: 33602886