Anti-Endomucin 抗体 [V.7C7.1] (ab106100)
![Immunocytochemistry/ Immunofluorescence - Anti-Endomucin antibody [V.7C7.1] (ab106100)](https://www.abcam.co.jp/ps/products/106/ab106100/Images/ab106100-314095-anti-endomucin-antibody-v7c71-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Endomucin antibody [V.7C7.1] (ab106100)")
Key features and details
- Rat monoclonal [V.7C7.1] to Endomucin
- Suitable for: IHC-Fr, IHC-P, IP, Flow Cyt, WB, IHC - Wholemount, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
製品の概要
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製品名
Anti-Endomucin antibody [V.7C7.1]
Endomucin 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [V.7C7.1] to Endomucin -
由来種
Rat -
アプリケーション
適用あり: IHC-Fr, IHC-P, IP, Flow Cyt, WB, IHC - Wholemount, ICC/IFmore details
適用なし: FuncS -
種交差性
交差種: Mouse
非交差種: Human -
免疫原
Other Immunogen Type corresponding to Mouse Endomucin. Mouse endomucin-IgG fusion protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
0.2 µm filtered solution -
ポリ/モノ
モノクローナル -
クローン名
V.7C7.1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106100の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| IHC-P | (4) |
Use at an assay dependent concentration.
|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| WB |
Use at an assay dependent concentration.
|
|
| IHC - Wholemount | (1) |
Use at an assay dependent concentration.
|
| ICC/IF |
Use a concentration of 1 µg/ml.
|
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. |
|
IHC - Wholemount
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. -
組織特異性
Expressed in heart, kidney and lung. -
翻訳後修飾
Highly O-glycosylated. Sialic acid-rich glycoprotein. -
細胞内局在
Secreted and Cell membrane. Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks. - Information by UniProt
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参照データベース
- Entrez Gene: 59308 Mouse
- SwissProt: Q9R0H2 Mouse
- Unigene: 27343 Mouse
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別名
- EMCN antibody
- EMCN2 antibody
- Endomucin 2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Endomucin antibody [V.7C7.1] (ab106100)
Immuno fluorescence staining of bEND3 cells with ab106100 at a concentration of 1 μg/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab106100 は 57 報の論文で使用されています。
- Himmels P et al. T cell-dependent bispecific antibodies alter organ-specific endothelial cell-T cell interaction. EMBO Rep 24:e55532 (2023). PubMed: 36621885
- Tang AT et al. Cell-autonomous requirement for ACE2 across organs in lethal mouse SARS-CoV-2 infection. PLoS Biol 21:e3001989 (2023). PubMed: 36745682
- Wu X et al. Generation of Conditional Knockout Alleles for PRUNE-1. Cells 12:N/A (2023). PubMed: 36831191
- Zhou T et al. Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution. Adv Sci (Weinh) 10:e2206296 (2023). PubMed: 36965031
- Panebianco C et al. Counteracting gemcitabine+nab-paclitaxel induced dysbiosis in KRAS wild type and KRASG12D mutated pancreatic cancer in vivo model. Cell Death Discov 9:116 (2023). PubMed: 37019893