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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Endo G 抗体 [EP1665Y] (ab76122)

Submit a review Q&A (1)References (7)
Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
  • Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
  • Anti-Endo G antibody [EP1665Y] (ab76122)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1665Y] to Endo G
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Endo G antibody [EP1665Y]
    Endo G 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1665Y] to Endo G

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide within Human Endo G (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • HepG2 whole cell lysate (ab7900)

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76122の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
特記事項
WB
1/2000 - 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, also has ribonuclease (RNase) and RNase H activities. Capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.

  • 配列類似性

    Belongs to the DNA/RNA non-specific endonuclease family.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Endo G antibody
      • ENDOG antibody
      • Endonuclease G antibody
      • Endonuclease G mitochondrial antibody
      • EndonucleaseG antibody
      • FLJ27463 antibody
      • mitochondrial antibody
      • Mitochondrial endonuclease G antibody
      • NUCG_HUMAN antibody
      see all

    画像

    • Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
      Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Endo G HAP1 cell lysate (20 µg)
      Lane 3: Mouse liver tissue lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab76122 observed at 29 kDa. Red - loading control, ab7291, observed at 52 kDa.

      ab76122 was shown to specifically react with Endo G when Endo G knockout samples were used. Wild-type and Endo G knockout samples were subjected to SDS-PAGE. ab76122 and ab7291 (loading control to alpha tubulin) were diluted 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
      Western blot - Anti-Endo G antibody [EP1665Y] (ab76122)
      Anti-Endo G antibody [EP1665Y] (ab76122) at 1/2000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 36 kDa
      Observed band size: 35 kDa why is the actual band size different from the predicted?

    • Anti-Endo G antibody [EP1665Y] (ab76122)
      Anti-Endo G antibody [EP1665Y] (ab76122)

    参考文献 (7)

    ab76122 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76122 は 7 報の論文で使用されています。

    • Barés G  et al. ENDOG Impacts on Tumor Cell Proliferation and Tumor Prognosis in the Context of PI3K/PTEN Pathway Status. Cancers (Basel) 13:N/A (2021). PubMed: 34359707
    • Blasco N  et al. Involvement of the mitochondrial nuclease EndoG in the regulation of cell proliferation through the control of reactive oxygen species. Redox Biol 37:101736 (2020). PubMed: 33032073
    • Liu H  et al. Copper Induces Oxidative Stress and Apoptosis in the Mouse Liver. Oxid Med Cell Longev 2020:1359164 (2020). PubMed: 32411316
    • Zhu T  et al. TRPM2 channel regulates cytokines production in astrocytes and aggravates brain disorder during lipopolysaccharide-induced endotoxin sepsis. Int Immunopharmacol 75:105836 (2019). PubMed: 31450153
    • Wei Q  et al. The mitochondrial pathway is involved in sodium fluoride (NaF)-induced renal apoptosis in mice. Toxicol Res (Camb) 7:792-808 (2018). PubMed: 30310657
    • Yang S  et al. AKT2 Blocks Nucleus Translocation of Apoptosis-Inducing Factor (AIF) and Endonuclease G (EndoG) While Promoting Caspase Activation during Cardiac Ischemia. Int J Mol Sci 18:N/A (2017). PubMed: 28272306
    • Pardo R  et al. EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis. Endocrinology 157:3873-3887 (2016). Mouse . PubMed: 27547848

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    I have purchased ab76122 and it is not working in my western blotting;

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge for one vial of ab7900replacement with the order number 1148322.

    Expect to receive this product on Tuesday August 21. To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

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