Anti-EN2 抗体 (ab45867)
Key features and details
- Goat polyclonal to EN2
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-EN2 antibody
EN2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to EN2 -
由来種
Goat -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Dog
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免疫原
Synthetic peptide:
C-NHSTTAKEGKSDSE
, corresponding to C terminal amino acids 320-333 of Human EN2 -
ポジティブ･コントロール
- WB: Mouse brain lysate; ICC: HEK293 and U2OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45867の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
A 1 hour primary incubation at room temperature is recommended for this product. |
|
| ICC |
Use a concentration of 10 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). A 1 hour primary incubation at room temperature is recommended for this product. |
|
ICC
Use a concentration of 10 µg/ml. |
ターゲット情報
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関連性
EN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 2020 Human
- Entrez Gene: 13799 Mouse
- Entrez Gene: EN2 Rat
- Omim: 131310 Human
- SwissProt: Q05917 Chicken
- SwissProt: P19622 Human
- SwissProt: P09066 Mouse
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別名
- AUTS 1 antibody
- AUTS1 antibody
- EN 2 antibody
see all
画像
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Western blot - Anti-EN2 antibody (ab45867)Anti-EN2 antibody (ab45867) at 1 µg/ml + Mouse brain lysate at 35 µg
Predicted band size: 34 kDa -
Immunocytochemistry - Anti-EN2 antibody (ab45867)Immunocytochemistry analysis of U2OS cells labelling EN2 with ab45867 at 10 μg/mL. Cells fixed with paraformaldehyde, and permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor 488 secondary antibody (2 μg/mL), showing nuclear staining (green). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).
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Immunocytochemistry - Anti-EN2 antibody (ab45867)Immunocytochemistry analysis of HEK293 cells labelling EN2 with ab45867 at 10 μg/mL. Cells fixed with paraformaldehyde, and permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor 488 secondary antibody (2 μg/mL), showing nuclear staining (green). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab45867 は 7 報の論文で使用されています。
- Cao Y et al. Engrailed-2 promotes a malignant phenotype of esophageal squamous cell carcinoma through upregulating the expression of pro-oncogenic genes. PeerJ 8:e8662 (2020). PubMed: 32117645
- Li T et al. Engrailed 2 (EN2) acts as a glioma suppressor by inhibiting tumor proliferation/invasion and enhancing sensitivity to temozolomide. Cancer Cell Int 20:65 (2020). PubMed: 32158355
- Li Y et al. EN2 as an oncogene promotes tumor progression via regulating CCL20 in colorectal cancer. Cell Death Dis 11:604 (2020). PubMed: 32732864
- Zhang X et al. Retinal defects in mice lacking the autism-associated gene Engrailed-2. Neuroscience 408:177-190 (2019). PubMed: 30980901
- Provenzano G et al. Hippocampal dysregulation of neurofibromin-dependent pathways is associated with impaired spatial learning in engrailed 2 knock-out mice. J Neurosci 34:13281-8 (2014). Mouse . PubMed: 25274808
- Guan R et al. A novel protein is lower expressed in renal cell carcinoma. Int J Mol Sci 15:7398-408 (2014). WB . PubMed: 24786097
- Morgan R et al. Engrailed-2 (EN2): a tumor specific urinary biomarker for the early diagnosis of prostate cancer. Clin Cancer Res 17:1090-8 (2011). WB ; Human . PubMed: 21364037