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AB250857

Anti-EMILIN1 抗体 [EPR14678] - BSA and Azide free

Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal EMILIN1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

別名を表示する

EMI, EMILIN1, EMILIN-1, Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1

2 Images
Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free (AB250857)
  • IP

Supplier Data

Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free (AB250857)

This data was developed using ab185953, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human fetal kidney tissue lysate labeling EMILIN1 with ab185953 at a 1/50 dilution. Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - N-terminal (<a href='/products/primary-antibodies/emilin1-antibody-epr14678-n-terminal-ab185953'>ab185953</a>)

Predicted band size: 107 kDa

false

Western blot - Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free (AB250857)
  • WB

Supplier Data

Western blot - Anti-EMILIN1 antibody [EPR14678] - BSA and Azide free (AB250857)

This data was developed using ab185953, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EMILIN1 antibody [EPR14678] - N-terminal (<a href='/products/primary-antibodies/emilin1-antibody-epr14678-n-terminal-ab185953'>ab185953</a>) at 1/50000 dilution

Lane 1:

Human uterus tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Lane 3:

Human colon cancer tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 107 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Unconjugated

    Anti-EMILIN1 antibody [EPR14678] - N-terminal

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14678

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

ab250857 is the carrier-free version of ab185953.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

EMILIN1 also known as elastin microfibril interface located protein 1 is a glycoprotein that plays a role in the extracellular matrix. The protein has a molecular mass of approximately 120 kDa. It is expressed in several tissues with high levels observed in vascular tissues skin and other connective tissues. EMILIN1 interacts with other matrix proteins which helps maintain the structural integrity of tissues.
Biological function summary

EMILIN1 contributes to maintaining elastogenesis and modulating cell adhesion by interacting with integrins. It forms part of the extracellular matrix where it supports elastin fibers which gives elasticity to tissues. EMILIN1 also influences cell behavior by modulating the signaling pathways through its binding to integrins. The proper function of EMILIN1 is important for tissue elasticity and cell migration.

Pathways

EMILIN1 is integral to the Notch signaling and TGF-β pathways. It interacts with Notch receptors affecting cell differentiation and proliferation. Additionally its interplay with TGF-β signaling regulates extracellular matrix organization and angiogenesis. These connections link EMILIN1 to proteins like TGF-β and Notch receptors highlighting its role in maintaining cellular and tissue homeostasis.

Defects or altered expression of EMILIN1 have been implicated in cardiovascular diseases and skin disorders. It has connections to proteins such as integrins and TGF-β which are also involved in these conditions. In cardiovascular disorders EMILIN1 dysfunction can lead to improper vessel formation and elasticity contributing to hypertension and atherosclerosis. Skin disorders involving EMILIN1 may present as conditions with compromised elastic fiber architecture such as cutis laxa.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Involved in elastic and collagen fibers formation. It is required for EFEMP2 deposition into the extracellular matrix, and collagen network assembly and cross-linking via protein-lysine 6-oxidase/LOX activity (PubMed : 36351433). May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved in the processes that regulate vessel assembly. Has cell adhesive capacity.
See full target information EMILIN1
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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組合せ製品

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