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リコンビナントRabMAb

Anti-Emi1 抗体 [EPR15320] (ab184950)

Reviews (2) Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320] (ab184950)
  • Western blot - Anti-Emi1 antibody [EPR15320] (ab184950)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] (ab184950)
  • Flow Cytometry (Intracellular) - Anti-Emi1 antibody [EPR15320] (ab184950)
  • Anti-Emi1 antibody [EPR15320] (ab184950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15320] to Emi1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Emi1 antibody [EPR15320]
    Emi1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15320] to Emi1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2 and HeLa cells and cell lysates; 293 and K562 cell lysates; Human ovarian carcinoma tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR15320

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184950の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
ICC/IF
1/250.
WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.

  • 配列類似性

    Contains 1 F-box domain.
    Contains 1 IBR-type zinc finger.

  • 発生段階

    Accumulates in late G1 phase, levels rise during S phase and drop in early mitosis.

  • ドメイン

    The C-terminal region is required for inhibition of APC activity.

  • 翻訳後修飾

    Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression.

  • 細胞内局在

    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Early mitotic inhibitor 1 antibody
      • Emi 1 antibody
      • EMI1 antibody
      • F box only protein 5 antibody
      • F box protein 5 antibody
      • F box protein Fbx 5 antibody
      • F box protein Fbx5 antibody
      • F-box only protein 5 antibody
      • FBX5 antibody
      • FBX5_HUMAN antibody
      • Fbxo31 antibody
      • fbxo5 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320] (ab184950)
      Immunocytochemistry/ Immunofluorescence - Anti-Emi1 antibody [EPR15320] (ab184950)

      Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling Emi1 with ab184950 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody. The slide on the right is counter stained with Dapi (blue).

    • Western blot - Anti-Emi1 antibody [EPR15320] (ab184950)
      Western blot - Anti-Emi1 antibody [EPR15320] (ab184950)
      All lanes : Anti-Emi1 antibody [EPR15320] (ab184950) at 1/5000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 50 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] (ab184950)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Emi1 antibody [EPR15320] (ab184950)

      Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Emi1 with ab184950 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Emi1 antibody [EPR15320] (ab184950)
      Flow Cytometry (Intracellular) - Anti-Emi1 antibody [EPR15320] (ab184950)

      Intracellular flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Emi1with ab184950 at 1/180 dilution (red). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (green). 

       

       

    • Anti-Emi1 antibody [EPR15320] (ab184950)
      Anti-Emi1 antibody [EPR15320] (ab184950)

    参考文献 (1)

    ab184950 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184950 は 1 報の論文で使用されています。

    • Fan X  et al. Licochalcone A induces cell cycle arrest and apoptosis via suppressing MAPK signaling pathway and the expression of FBXO5 in lung squamous cell cancer. Oncol Rep 50:N/A (2023). PubMed: 37859622

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry abreview for Anti-Emi1 antibody [EPR15320]

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    投稿 Jan 14 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Emi1 antibody [EPR15320]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    投稿 Nov 03 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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