Anti-Emerin 抗体 (ab40688)
Key features and details
- Rabbit polyclonal to Emerin
- Suitable for: IP, ICC/IF, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Emerin antibody
Emerin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Emerin -
由来種
Rabbit -
アプリケーション
適用あり: IP, ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Emerin.
Immunogen の所有権に関して (Peptide available as ab40723) -
ポジティブ･コントロール
- WB: HEK-293T, HAP1 and HeLa whole cell lysates; HeLa nuclear lysate. IHC-P: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40688の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use a concentration of 5 µg/ml.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 25, 29 kDa).
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| 特記事項 |
|---|
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IP
Use a concentration of 5 µg/ml. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 25, 29 kDa). |
ターゲット情報
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機能
Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C. -
組織特異性
Skeletal muscle, heart, colon, testis, ovary and pancreas. -
関連疾患
Defects in EMD are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1) [MIM:310300]. A degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. -
配列類似性
Contains 1 LEM domain. -
翻訳後修飾
Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle. -
細胞内局在
Nucleus inner membrane. Nucleus outer membrane. Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non-farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 399681 Cow
- Entrez Gene: 2010 Human
- Entrez Gene: 13726 Mouse
- Entrez Gene: 25437 Rat
- Omim: 300384 Human
- SwissProt: P50402 Human
- SwissProt: O08579 Mouse
- SwissProt: Q63190 Rat
see all -
別名
- EDMD antibody
- Emd antibody
- EMD_HUMAN antibody
see all
画像
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Western blot - Anti-Emerin antibody (ab40688)All lanes : Anti-Emerin antibody (ab40688) at 1 µg/ml
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : EMD knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25, 29 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab40688 observed at 35 kDa. Red - loading control ab8245 observed at 37 kDa.
ab40688 Anti-Emerin antibody was shown to specifically react with Emerin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266336 (knockout cell lysate ab257423) was used. Wild-type and Emerin knockout samples were subjected to SDS-PAGE. ab40688 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Emerin antibody (ab40688)All lanes : Anti-Emerin antibody (ab40688) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Emerin knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25, 29 kDaLanes 1 - 3: Merged signal (red and green). Green - ab40688 observed at 35 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab40688 was shown to recognize Emerin in wild-type HAP1 cells as signal was lost at the expected MW in Emerin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Emerin knockout samples were subjected to SDS-PAGE. Ab40688 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Emerin antibody (ab40688)All lanes : Anti-Emerin antibody (ab40688) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 :Jurkat whole cell lysate (ab7899)
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 25, 29 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Emerin antibody (ab40688)ICC/IF image of ab40688 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40688, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Immunoprecipitation - Anti-Emerin antibody (ab40688)Emerin was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Emerin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab40688.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 33kDa; Emerin;non specific bands - 52 and 60kDa: We are unsure as to the identity of this extra band.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab40688 は 14 報の論文で使用されています。
- Fernandez A et al. Emerin self-assembly and nucleoskeletal coupling regulate nuclear envelope mechanics against stress. J Cell Sci 135:N/A (2022). PubMed: 35178558
- Raina K & Rao BJ Mammalian nuclear speckles exhibit stable association with chromatin: a biochemical study. Nucleus 13:58-73 (2022). PubMed: 35220893
- Zhang C et al. ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma. J Cell Mol Med 26:5078-5094 (2022). PubMed: 36071546
- Horiguchi M et al. Characterizing the degeneration of nuclear membrane and mitochondria of adipose-derived mesenchymal stem cells from patients with type II diabetes. J Cell Mol Med 25:4298-4306 (2021). PubMed: 33759360
- Zhang T et al. Influenza Virus Z-RNAs Induce ZBP1-Mediated Necroptosis. Cell 180:1115-1129.e13 (2020). PubMed: 32200799