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リコンビナントRabMAb

Anti-EMC4 抗体 [EPR15080] (ab184544)

Submit a review Submit a question References (5)
Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)
  • Anti-EMC4 antibody [EPR15080] (ab184544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15080] to EMC4
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-EMC4 antibody [EPR15080]
    EMC4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15080] to EMC4

  • 由来種

    Rabbit

  • 特異性

    Based on the sequence analysis, this antibody recognizes three isoforms with the predicted MWs of 20KDa, 17KDa and 15KDa, respectively.

  • アプリケーション

    適用あり: IP, WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • 293, SH-SY5Y, U87-MG and HepG2 cell lysates; Human brain and ovarian carcinoma tissues; mouse liver tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR15080

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184544の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/50 - 1/70.
WB
1/10000 - 1/50000. Detects a band of approximately 20, 17 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IP
1/50 - 1/70.
WB
1/10000 - 1/50000. Detects a band of approximately 20, 17 kDa (predicted molecular weight: 20 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

画像

  • Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
    Western blot - Anti-EMC4 antibody [EPR15080] (ab184544)
    All lanes : Anti-EMC4 antibody [EPR15080] (ab184544) at 1/10000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 17,20 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EMC4 antibody [EPR15080] (ab184544)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EMC4 with ab184544 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)
    Immunoprecipitation - Anti-EMC4 antibody [EPR15080] (ab184544)

    Western blot analysis of EMC4 in HepG2 cell lysate immunoprecipitated using ab184544 at 1/50 dilution (Lane 1). Lane 2: PBS.

    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Anti-EMC4 antibody [EPR15080] (ab184544)
    Anti-EMC4 antibody [EPR15080] (ab184544)

参考文献 (5)

ab184544 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184544 は 5 報の論文で使用されています。

  • Tang X  et al. EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly. iScience 26:105667 (2023). PubMed: 36624844
  • Campos RK  et al. Ribosomal stalk proteins RPLP1 and RPLP2 promote biogenesis of flaviviral and cellular multi-pass transmembrane proteins. Nucleic Acids Res 48:9872-9885 (2020). PubMed: 32890404
  • Miller-Vedam LE  et al. Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients. Elife 9:N/A (2020). PubMed: 33236988
  • Coelho JPL  et al. A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration. Nat Commun 10:672 (2019). PubMed: 30737405
  • Tian S  et al. Proteomic Analysis Identifies Membrane Proteins Dependent on the ER Membrane Protein Complex. Cell Rep 28:2517-2526.e5 (2019). PubMed: 31484065

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