Anti-ELYS 抗体 (ab14431)
Key features and details
- Rabbit polyclonal to ELYS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ELYS antibody
ELYS 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ELYS -
由来種
Rabbit -
特異性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Orangutan
-
免疫原
Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa2220 and aa2302 of human ELYS using the numbering given in entry AAN65622.1 (GeneID 25909).
-
ポジティブ･コントロール
- HeLa cells nuclear extract.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 8% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was purified using an epitope specific to ELYS immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14431の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 272 kDa.
|
| 特記事項 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 272 kDa. |
ターゲット情報
-
機能
Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis. -
配列類似性
Belongs to the ELYS family.
Contains 1 A.T hook DNA-binding domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus matrix. Chromosome > centromere > kinetochore. Nucleus > nucleoplasm. Nucleus > nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase. - Information by UniProt
-
参照データベース
- Entrez Gene: 25909 Human
- Omim: 610853 Human
- SwissProt: Q8IZA4 Human
- SwissProt: Q8WYP5 Human
- Unigene: 300887 Human
-
製品の状態
There are 2 isoforms produced by alternative splicing. -
別名
- ahctf1 antibody
- AT hook containing transcription factor 1 antibody
- DKFZp434N093 antibody
see all
画像
-
Western blot - Anti-ELYS antibody (ab14431)All lanes : Anti-ELYS antibody (ab14431) at 0.5 µg/ml
Lane 1 : Cell lysates prepared from Hela cells at 10 µg/ml
Lane 2 : Cell lysates prepared from Hela cells at 20 µg/ml
Predicted band size: 272 kDaab14431 at 0.5mg/ml staining human ELYS by Western blot using chemiluminescence with exposure times of 3-5 minutes. Left lane 10mg HeLa nuclear extract, right lane 20mg.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab14431 は 2 報の論文で使用されています。
- Kuiper EFE et al. The chaperone DNAJB6 surveils FG-nucleoporins and is required for interphase nuclear pore complex biogenesis. Nat Cell Biol 24:1584-1594 (2022). PubMed: 36302971
- Suzuki Y et al. Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg. Sci Rep 9:8461 (2019). PubMed: 31186495