Anti-ELMO2 抗体 (ab2240)
Key features and details
- Goat polyclonal to ELMO2
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ELMO2 antibody -
製品の詳細
Goat polyclonal to ELMO2 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WB, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Dog, Pig
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免疫原
Synthetic peptide: PKEPSSYDFVYHYG, corresponding to C terminal amino acids 707-720 of Human ELMO2.
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ポジティブ･コントロール
- Flow Cyt: A549 cells ICC/IF: U2OS and A549 cells WB: Jurkat, HEK293, RAW264.7 and KNRK cell lysate; Human cerebellum and rat brain tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2240の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 - 3 µg/ml.
1 hour primary incubation is recommended for this product. |
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| Flow Cyt |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 - 3 µg/ml. 1 hour primary incubation is recommended for this product. |
|
Flow Cyt
Use a concentration of 10 µg/ml. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. -
組織特異性
Widely expressed, with a higher expression in skeletal muscle, kidney and placenta. -
配列類似性
Contains 1 ELMO domain.
Contains 1 PH domain. -
細胞内局在
Cytoplasm. Cytoplasm > cytosol. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 508361 Cow
- Entrez Gene: 63916 Human
- Entrez Gene: 140579 Mouse
- Entrez Gene: 362271 Rat
- Omim: 606421 Human
- SwissProt: A4FUD6 Cow
- SwissProt: Q96JJ3 Human
- SwissProt: Q8BHL5 Mouse
see all -
別名
- CED 12 antibody
- Ced 12 homolog 2 antibody
- CED 12 homolog A antibody
see all
画像
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Western blot - Anti-ELMO2 antibody (ab2240)All lanes : Anti-ELMO2 antibody (ab2240) at 0.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : RAW264.7 cell lysate
Lane 4 : KNRK cell lysateDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (ab2240)Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL). -
Flow Cytometry - Anti-ELMO2 antibody (ab2240)Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Western blot - Anti-ELMO2 antibody (ab2240)All lanes : Anti-ELMO2 antibody (ab2240) at 0.5 µg/ml
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 35 µg per lane.Detected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (ab2240)Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab2240 は 7 報の論文で使用されています。
- Wang Y et al. ELMO2 association with Gai2 regulates pancreatic cancer cell chemotaxis and metastasis. PeerJ 8:e8910 (2020). PubMed: 32292657
- Hetmanski JHR et al. Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration. Dev Cell 51:460-475.e10 (2019). PubMed: 31607653
- Toret CP et al. A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion. J Cell Biol 204:265-79 (2014). ICC/IF, WB ; Drosophila melanogaster . PubMed: 24446484
- Toret CP et al. An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion. J Cell Biol 207:577-87 (2014). PubMed: 25452388
- Harada K et al. Ephexin4 and EphA2 mediate resistance to anoikis through RhoG and phosphatidylinositol 3-kinase. Exp Cell Res 317:1701-13 (2011). WB ; Human . PubMed: 21621533
- Hiramoto-Yamaki N et al. Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism. J Cell Biol 190:461-77 (2010). WB ; Human . PubMed: 20679435
- Gekonge BN et al. Signal transduction induced by apoptotic cells inhibits HIV transcription in monocytes/macrophages. J Leukoc Biol 80:953-60 (2006). WB . PubMed: 16885500