AB2239

Anti-ELMO1 抗体

Name: Anti-ELMO1 抗体 (ab2239) | Abcam
Brand: Abcam Limited
SKU: AB2239
Price: 82000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-ELMO1 antibody

BOND RX™ Validated
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(2 Reviews)

(14 Publications)

Goat Polyclonal ELMO1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human ELMO1 aa 700 to C-terminus.

別名を表示する

KIAA0281, ELMO1, Engulfment and cell motility protein 1, Protein ced-12 homolog

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELMO1 antibody (AB2239)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELMO1 antibody (AB2239)

IHC image of ab2239 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2239, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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Western blot - Anti-ELMO1 antibody (AB2239)

ab2239 staining (1μg/ml) of Jurkat lysate (RIPA buffer, 35μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab2239 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-ELMO1 antibody (ab2239)

Predicted band size: 84 kDa

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Supplier Data

Western blot - Anti-ELMO1 antibody (AB2239)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-ELMO1 antibody (ab2239) at 0.3 µg/mL

Lane 1:

Human Frontal Cortex cell lysate (35µg protein in RIPA buffer)

Lane 2:

Mouse cell lysate (35µg protein in RIPA buffer)

Lane 3:

Rat cell lysate (35µg protein in RIPA buffer)

Predicted band size: 84 kDa

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Key facts

宿主種

Goat

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human ELMO1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92556

特異性

This antibody is expected to recognise both human isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa)." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa)." }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa)." }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

バッファー組成

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ELMO1 also known as Engulfment and Cell Motility 1 protein functions in the regulation of cell migration and cytoskeletal reorganization. It possesses a molecular mass of approximately 82 kDa. This protein expresses across many tissues including the brain lung and kidney indicating widespread roles in different cellular processes. Researchers frequently study ELMO1 due to its involvement in essential cellular functions linking to motility and immune responses.

Biological function summary

The protein ELMO1 participates in the regulation of Rac signaling pathways through the formation of complexes with DOCK family proteins. This complex formation contributes to the activation of Rac a small GTPase critical for actin cytoskeleton dynamics. ELMO1 affects processes such as phagocytosis and cell migration by modulating cytoskeletal configurations showing its role in the cellular machinery controlling cell shape and movement.

Pathways

The ELMO1 protein contributes to the Rac1 signaling and the phagocytosis pathway. These pathways play essential roles in integrating extracellular signals to promote cytoskeletal rearrangement and cellular movement. ELMO1 interacts with proteins like DOCK180 to facilitate these pathways. Through these interactions ELMO1 orchestrates cellular responses including actin polymerization and membrane extensions enabling effective cellular reactions to external stimuli.

Research links ELMO1 to conditions such as diabetic nephropathy and inflammatory disorders. Elevated levels of ELMO1 expression correlate with increased diabetic nephropathy progression implicating its role in this disease. In inflammatory disorders ELMO1 coordinates with proteins like Rac1 to influence macrophage function and other immune cell behaviors. These interactions show ELMO1 as an important factor in disease mechanisms where cell migration and immune responses play a significant role.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

See full target information ELMO1

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文献 (14)

Recent publications for all applications. Explore the full list and refine your search

Annals of dermatology 35:439-450 PubMed38086358

2023

Overexpression of Dock180 and Elmo1 in Melanoma is Associated with Cell Survival and Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon Jin Lee,Yu Sung Choi,Sooyoung Kim,Jae Young Heo,Dong Sung Kim,Ki Dam Kim,Seung Min Nam,Hae Seon Nam,Sang Han Lee,Dongsic Choi,Moon Kyun Cho

Cells 9: PubMed32708184

2020

The Lipid Receptor G2A (GPR132) Mediates Macrophage Migration in Nerve Injury-Induced Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Tabea Osthues,Béla Zimmer,Vittoria Rimola,Kevin Klann,Karin Schilling,Praveen Mathoor,Carlo Angioni,Andreas Weigert,Gerd Geisslinger,Christian Münch,Klaus Scholich,Marco Sisignano

Nature communications 10:4456 PubMed31575859

2019

Phosphatidylserine on viable sperm and phagocytic machinery in oocytes regulate mammalian fertilization.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia M Rival,Wenhao Xu,Laura S Shankman,Sho Morioka,Sanja Arandjelovic,Chang Sup Lee,Karen M Wheeler,Ryan P Smith,Lisa B Haney,Brant E Isakson,Scott Purcell,Jeffrey J Lysiak,Kodi S Ravichandran

Cellular and molecular life sciences : CMLS 77:161-177 PubMed31161284

2019

Elmo1 function, linked to Rac1 activity, regulates peripheral neuronal numbers and myelination in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Aya Mikdache,Laura Fontenas,Shahad Albadri,Celine Revenu,Julien Loisel-Duwattez,Emilie Lesport,Cindy Degerny,Filippo Del Bene,Marcel Tawk

Cell communication and signaling : CCS 13:35 PubMed26205662

2015

Tyr724 phosphorylation of ELMO1 by Src is involved in cell spreading and migration via Rac1 activation.

Applications

Species

Unspecified reactive species

Yoshinori Makino,Masumi Tsuda,Yusuke Ohba,Hiroshi Nishihara,Hirofumi Sawa,Kazuo Nagashima,Shinya Tanaka

The Journal of cell biology 207:577-87 PubMed25452388

2014

An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher P Toret,Caitlin Collins,W James Nelson

PloS one 9:e111568 PubMed25360637

2014

ELMO1 is upregulated in AML CD34+ stem/progenitor cells, mediates chemotaxis and predicts poor prognosis in normal karyotype AML.

Applications

Species

Human

Marta E Capala,Edo Vellenga,Jan Jacob Schuringa

Nature genetics 45:478-86 PubMed23525077

2013

Exome and whole-genome sequencing of esophageal adenocarcinoma identifies recurrent driver events and mutational complexity.

Applications

Species

Unspecified reactive species

Austin M Dulak,Petar Stojanov,Shouyong Peng,Michael S Lawrence,Cameron Fox,Chip Stewart,Santhoshi Bandla,Yu Imamura,Steven E Schumacher,Erica Shefler,Aaron McKenna,Scott L Carter,Kristian Cibulskis,Andrey Sivachenko,Gordon Saksena,Douglas Voet,Alex H Ramos,Daniel Auclair,Kristin Thompson,Carrie Sougnez,Robert C Onofrio,Candace Guiducci,Rameen Beroukhim,Zhongren Zhou,Lin Lin,Jules Lin,Rishindra Reddy,Andrew Chang,Rodney Landrenau,Arjun Pennathur,Shuji Ogino,James D Luketich,Todd R Golub,Stacey B Gabriel,Eric S Lander,David G Beer,Tony E Godfrey,Gad Getz,Adam J Bass

The Journal of biological chemistry 286:37615-24 PubMed21900250

2011

The RhoG/ELMO1/Dock180 signaling module is required for spine morphogenesis in hippocampal neurons.

Applications

ICC/IF, WB

Species

Rat, Rat

Jeong-Yoon Kim,Mi Hee Oh,Laura P Bernard,Ian G Macara,Huaye Zhang

The Journal of biological chemistry 283:26538-47 PubMed18662984

2008

Parallel phosphatidylinositol 3-kinase (PI3K)-dependent and Src-dependent pathways lead to CXCL8-mediated Rac2 activation and chemotaxis.

Applications

IP, WB, ICC/IF

Species

Human, Human, Human

Jiqing Sai,Dayanidhi Raman,Yuxin Liu,John Wikswo,Ann Richmond

View all publications

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