Anti-ELL3 抗体 (ab67415)
Key features and details
- Mouse polyclonal to ELL3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ELL3 antibody -
製品の詳細
Mouse polyclonal to ELL3 -
由来種
Mouse -
特異性
Reactive against mammalian transfected lysate. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human ELL3 aa 1-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ･コントロール
- Transfected 293T lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67415の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa).
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 45 kDa). |
ターゲット情報
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関連性
ELL3 is a testis-specific RNA polymerase II elongation factor which increases the catalytic rate of transcription elongation. ELL3 is approximately 50% similar to both ELL and ELL2. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 80237 Human
- Omim: 609885 Human
- SwissProt: Q9HB65 Human
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別名
- Elongation Factor RNA Polymerase II-Like 3 antibody
- RNA polymerase II elongation factor ELL3 antibody
画像
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Western blot - Anti-ELL3 antibody (ab67415)All lanes : Anti-ELL3 antibody (ab67415) at 1/500 dilution
Lane 1 : ELL3 transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 45 kDa
Observed band size: 43.78 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (3)
ab67415 は 3 報の論文で使用されています。
- Chen Y et al. The Expression and Roles of the Super Elongation Complex in Mouse Cochlear Lgr5+ Progenitor Cells. Front Cell Neurosci 15:735723 (2021). PubMed: 34658793
- Lee JY et al. Ell3 functions as a critical decision maker at the crossroad between stem cell senescence and apoptosis. Stem Cell Res Ther 10:32 (2019). PubMed: 30654843
- Ahn HJ et al. Ell3 stabilizes p53 following CDDP treatment via its effects on ubiquitin-dependent and -independent proteasomal degradation pathways in breast cancer cells. Oncotarget 6:44523-37 (2015). WB . PubMed: 26540344
