Anti-ELL2 抗体 (ab115027)
Key features and details
- Rabbit polyclonal to ELL2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ELL2 antibody -
製品の詳細
Rabbit polyclonal to ELL2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
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免疫原
Synthetic peptide, corresponding to a region within amino acids 400-450 of Human ELL2 (NP_036213.2).
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ポジティブ･コントロール
- HeLa whole cell lysate (ab150035)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab115027 was affinity purified using an epitope specific to ELL2 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115027の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 72 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Elongation factor that can increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. -
配列類似性
Belongs to the ELL/occludin family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 22936 Human
- Entrez Gene: 309918 Rat
- Omim: 601874 Human
- SwissProt: O00472 Human
- Unigene: 192221 Human
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別名
- ELL related RNA polymerase II elongation factor antibody
- Ell2 antibody
- ELL2_HUMAN antibody
see all
画像
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Western blot - Anti-ELL2 antibody (ab115027)All lanes : Anti-ELL2 antibody (ab115027) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-ELL2 antibody (ab115027)Detection of ELL2 by Western Blot of Immunprecipitate.
ab115027, at 1 µg/ml, staining ELL2 in Hela whole cell lysate immunoprecipitated using ab115027 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab115027 は 1 報の論文で使用されています。
- Kuzmina A et al. Super elongation complex promotes early HIV transcription and its function is modulated by P-TEFb. Transcription 8:133-149 (2017). PubMed: 28340332