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AB186430

Anti-Elav-type RNA-binding protein ETR3 抗体 [EPR13374-2]

Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2]

5

(2 Reviews)

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(7 Publications)

Rabbit Recombinant Monoclonal Elav-type RNA-binding protein ETR3 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

別名を表示する

BRUNOL3, CUGBP2, ETR3, NAPOR, CELF2, CUGBP Elav-like family member 2, CELF-2, Bruno-like protein 3, CUG triplet repeat RNA-binding protein 2, CUG-BP- and ETR-3-like factor 2, ELAV-type RNA-binding protein 3, Neuroblastoma apoptosis-related RNA-binding protein, RNA-binding protein BRUNOL-3, CUG-BP2, ETR-3, hNAPOR

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

ab186430 staining Elav-type RNA-binding protein ETR3 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilised with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Negative control 1 : PBS only.

Flow Cytometry (Intracellular) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3with ab186430 at 1/270 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • IP

Supplier Data

Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

Western blot analysis of Elav-type RNA-binding protein ETR3 in HeLa cell lysate immunoprecipitated with ab186430 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of HeLa lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430)

Predicted band size: 54 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Elav-type RNA-binding protein ETR3 with ab186430 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • WB

Supplier Data

Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

All lanes:

Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)
  • WB

Supplier Data

Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

All lanes:

Western blot - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (ab186430) at 1/5000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

関連する標識済み抗体及び組成の異なる製品 (2)

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    Alexa Fluor® 647 Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2]

  • Carrier free

    Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR13374-2

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, WB, ICC/IF, Flow Cyt (Intra), IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/270", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Elav-type RNA-binding protein ETR3 also known as CELF1 (CUGBP Elav-like family member 1) is involved in the regulation of pre-mRNA alternative splicing translation and stability. The protein has a mass of approximately 52 kDa. ETR3 is expressed in several tissues with higher levels found in the heart skeletal muscles and brain. These tissues are indicative of its important role in post-transcriptional mechanism. As a member of the ELAV (embryonic lethal abnormal vision)-like proteins ETR3 contains three RNA recognition motifs which mediate its binding to RNA.
Biological function summary

ETR3 influences various cellular processes by participating in RNA processing and nuclear-cytoplasmic transport. It forms part of ribonucleoprotein complexes that interact with target RNA impacting splicing decisions and gene expression levels. ETR3's activity is important for muscle and neuronal function helping modulate the expression of genes vital for muscle contraction and neuronal communication. Through its role as an RNA-binding regulator it can affect cell differentiation and growth.

Pathways

ETR3 plays significant roles in the regulation of alternative splicing and mRNA decay pathways. It interacts with several other splicing and mRNA degradation factors such as hnRNPH (heterogeneous nuclear ribonucleoprotein H) and PABPN1 (poly(A) binding protein nuclear 1). These interactions help control the turnover and translation of mRNA establishing a balance of protein synthesis important for maintaining cellular homeostasis. By influencing these pathways ETR3 impacts cardiac and neural-specific gene expression demonstrating its role in tissue-specific regulation.

ETR3 is linked to myotonic dystrophy and cardiac arrhythmias. ETR3 misregulation leads to disruptions in alternative splicing of specific mRNAs contributing to the pathophysiology of these conditions. The relationship between ETR3 and proteins such as MBNL1 (Muscleblind-like splicing regulator 1) in myotonic dystrophy is particularly significant. Both proteins regulate alternative splicing and their misregulated expression in disease highlights an important area for potential therapeutic interventions. The association of ETR3 with such disorders highlights its relevance in maintaining normal cellular and physiological functions.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic, but not adult, skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts both as an activator and as a repressor of a pair of coregulated exons : promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences, in particular UG repeat and UGUU motifs. Binds to apoB mRNA, specifically to AU-rich sequences located immediately upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity). May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and, with CELF1, negatively regulates the processing to mature miRNA (PubMed : 28431233).
See full target information CELF2
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes research 2024:6942156 PubMed38282657

2024

Huaju Xiaoji Formula Regulates ERS-lncMGC/miRNA to Enhance the Renal Function of Hypertensive Diabetic Mice with Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zeng Zhang,Xiaodong Fu,Fengzhu Zhou,Duanchun Zhang,Yanqiu Xu,Zhaohua Fan,Shimei Wen,Yanting Shao,Zheng Yao,Yanming He

Cell cycle (Georgetown, Tex.) 22:117-130 PubMed35941836

2022

ELK1-Induced upregulation of long non-coding TNK2-AS1 promotes the progression of acute myeloid leukemia by EZH2-mediated epigenetic silencing of CELF2.

Applications

Unspecified application

Species

Unspecified reactive species

Dongfang Guo,Airong Zhang,Meifang Suo,Ping Wang,Yile Liang

Scientific reports 11:3628 PubMed33574559

2021

GAS5 knockdown alleviates spinal cord injury by reducing VAV1 expression via RNA binding protein CELF2.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Xiaoxiao Xu,Junwei Pan,Shixin Zhao,Yu Li,Zhen Wang,Jiahao Yang,Xi Zhang,Yisheng Wang,Ming Liu

Carcinogenesis 41:377-389 PubMed31241130

2019

CELF2 suppresses non-small cell lung carcinoma growth by inhibiting the PREX2-PTEN interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Yiu To Yeung,Suyu Fan,Bingbing Lu,Shuying Yin,Sen Yang,Wenna Nie,Meixian Wang,Liting Zhou,Tiepeng Li,Xiang Li,Ann M Bode,Zigang Dong

World journal of gastroenterology 25:205-219 PubMed30670910

2019

Human antigen R mediated post-transcriptional regulation of inhibitors of apoptosis proteins in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ausra Lukosiute-Urboniene,Aldona Jasukaitiene,Giedre Silkuniene,Vidmantas Barauskas,Antanas Gulbinas,Zilvinas Dambrauskas

Cellular physiology and biochemistry : internation 48:1215-1229 PubMed30045014

2018

RNA Binding Proteins Control Transdifferentiation of Hepatic Stellate Cells into Myofibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Sihyung Wang,Youngmi Jung,Jeongeun Hyun,Matthew Friedersdorf,Seh-Hoon Oh,Jieun Kim,Richard T Premont,Jack D Keene,Anna Mae Diehl

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 101:406-413 PubMed29501762

2018

miR-615-3p promotes proliferation and migration and inhibits apoptosis through its potential target CELF2 in gastric cancer.

Applications

WB

Species

Unspecified reactive species

Jizhao Wang,Lin Liu,Yuchen Sun,Yumo Xue,Jingkun Qu,Shupei Pan,Huajing Li,Hangying Qu,Jiansheng Wang,Jia Zhang
View all publications
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代替製品

Primary Antibodies

AB179447

Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374] (AB179447)

  • 0
  • 0

組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Lab Essentials

Western blot - Anti-VEGFA antibody (AB46154)

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Anti-TGF beta 1 antibody [EPR21143]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

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Primary Antibodies

AB9549

Anti-CUG-BP1 antibody [3B1]

BOND RX™ Validated|Trial Size

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