Anti-Elafin/ESI 抗体 [EPR14631] (ab184972)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elafin/ESI antibody [EPR14631] (ab184972)](https://www.abcam.co.jp/ps/products/184/ab184972/Images/ab184972-541977-anti-elafin-esi-antibody-epr14631-bsa-and-azide-free-human-colon.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elafin/ESI antibody [EPR14631] (ab184972)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14631] to Elafin/ESI
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Elafin/ESI antibody [EPR14631]
Elafin/ESI 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14631] to Elafin/ESI -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- A431 cell lysate; Human recombinant Elafin/ESI protein. IHC-P: Human colon, Human esophagus
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14631 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184972の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 12, 14 kDa (predicted molecular weight: 12 kDa).
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 12, 14 kDa (predicted molecular weight: 12 kDa). |
ターゲット情報
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機能
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. -
配列類似性
Contains 1 WAP domain. -
ドメイン
Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5266 Human
- Omim: 182257 Human
- SwissProt: P19957 Human
- Unigene: 112341 Human
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別名
- Cementoin antibody
- ELAF_HUMAN antibody
- Elafin antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elafin/ESI antibody [EPR14631] (ab184972)
Immunohistochemical analysis of paraffin-embedded Human colon labelling Elafin/ESI with ab184972 at 1/5000 dilution (0.2 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on single cells in human colon.
The section was incubated with ab184972 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elafin/ESI antibody [EPR14631] (ab184972)](https://www.abcam.co.jp/ps/products/184/ab184972/Images/ab184972-541975-anti-elafin-esi-antibody-epr14631-bsa-and-azide-free-human-esophagus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elafin/ESI antibody [EPR14631] (ab184972)Immunohistochemical analysis of paraffin-embedded Human esophagus labelling Elafin/ESI with ab184972 at 1/5000 dilution (0.2 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human esophagus.
The section was incubated with ab184972 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: LeicaDS9800 (Bond™ Polymer Refine Detection). Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elafin/ESI antibody [EPR14631] (ab184972)](https://www.abcam.co.jp/ps/products/184/ab184972/Images/ab184972-541979-anti-elafin-esi-antibody-epr14631-bsa-and-azide-free-human-skeletal-muscle-negative-control.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elafin/ESI antibody [EPR14631] (ab184972)Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labelling Elafin/ESI with ab184972 at 1/5000 dilution (0.2 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection.
Negative control: No staining on human skeletal muscle.
The section was incubated with ab184972 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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![Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)](https://www.abcam.co.jp/ps/products/184/ab184972/Images/ab184972-217772-ab184972WB.jpg)
Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)Anti-Elafin/ESI antibody [EPR14631] (ab184972) at 1/2000 dilution + A431 cell lysate at 20 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa -
![Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)](https://www.abcam.co.jp/ps/products/184/ab184972/Images/ab184972-217771-ab184972WB2.jpg)
Western blot - Anti-Elafin/ESI antibody [EPR14631] (ab184972)Anti-Elafin/ESI antibody [EPR14631] (ab184972) at 1/10000 dilution + Human recombinant His-tagged Elafin/ESI protein (amino acids 23-117) at 0.01 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa -
![Anti-Elafin/ESI antibody [EPR14631] (ab184972)](https://www.abcam.co.jp/ps/products/184/ab184972/Images/ab184972-3-benefits-of-recombinant-antibodies.png)
Anti-Elafin/ESI antibody [EPR14631] (ab184972)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab184972 は 1 報の論文で使用されています。
- Wang X et al. Identification of potential miR‑155 target genes in epidermal immune microenvironment of atopic dermatitis patients and their inflammatory effects on HaCaT cells. Exp Ther Med 27:25 (2024). PubMed: 38125354