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リコンビナントRabMAb

Anti-eIF5A2 + eIF5A 抗体 [EPR7412-47] (ab126733)

Submit a review Q&A (1)References (1)
Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
  • Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7412-47] to eIF5A2 + eIF5A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-eIF5A2 + eIF5A antibody [EPR7412-47]
    eIF5A2 + eIF5A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7412-47] to eIF5A2 + eIF5A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human eIF5A2 + eIF5A aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary. The immunogen sequence is based upon a region of eIF5A2 that has high homology with eIF5A.
    Database link: Q9GZV4

  • ポジティブ･コントロール

    • HepG2 and Caco 2 cell lysates; Human ovarian carcinoma tissue; HepG2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab126733の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for IP.

ターゲット情報

画像

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

    ab126733 at 1/100 dilution, staining eIF5A2 + eIF5A in Formalin-fixed Paraffin-embedded Human ovarian carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

    ab126733, at 1/100 dilution, staining eIF5A2 + eIF5A in HepG2 cells by Immunofluorescence (green). DAPI counterstain (blue).

  • Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Western blot - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    All lanes : Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733) at 1/1000 dilution

    Lane 1 : Recombinant Human eIF5A protein (ab87457)
    Lane 2 : Recombinant Human eIF5A2 protein (ab99140)

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 17 kDa
    Observed band size: 15,20 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking buffer: 5% NFDM/TBST

  • OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    OI-RD Scanning - Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)
    Anti-eIF5A2 + eIF5A antibody [EPR7412-47] (ab126733)

参考文献 (1)

ab126733 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab126733 は 1 報の論文で使用されています。

  • Xu G  et al. Eukaryotic initiation factor 5A2 mediates hypoxia-induced autophagy and cisplatin resistance. Cell Death Dis 13:683 (2022). PubMed: 35931669

レビューと Q&A

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レビューを送る 質問を送る

Question

Dear Sir,
I have a question regarding antibodies against eIF5A-1 and eIF5A-2. Both proteins are very similar and it seems that some eIF5A-2 antibodies recognize eIF5A-1. I am wondering if you have antibodies that distinguish eIf5A-1 and eIF5A-2. I’ve look at your websites for all eIF5A-1 and eIF5A-2 antibodies (ex. Ab126733, ab150439…etc). In this case, monoclonal antibodies are better, but the peptide sequence is not shown. There are some differences towards C-terminus between these two protein sequences. Please let me know which products recognize only eIF5A-1 or eIF5A-2, if you have tested the specificity.

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Answer

Thank you for contacting us. The antibody ab150439 is tested and is very specific for eIF5A-2. Regarding eIF5A-1 (otherwise known as eIF5A) many of these antibodies have some amount of cross-reactivity, although one predicted to be least likely to cross-react is ab106782, based on homology with the immunogen.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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