Anti-eIF4G1 antibody [EPR30302-554] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-eIF4G1 antibody [EPR30302-554] - BSA and Azide free (AB324959)

This data was developed using ab324948, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9819442; PMID : 36897548).

In lanes 1-4, the lysates were stored at -80°C prior to Western Blotting. The bands beneath the target band (260 kDa) are likely to be degradation products. In lanes 5-6, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lanes 1-2 : 48 seconds; Lanes 3-4 : 180 seconds; Lanes 5-6 : 15 seconds

All lanes:

Western blot - Anti-eIF4G1 antibody [EPR30302-554] (<a href='/products/primary-antibodies/eif4g1-antibody-epr30302-554-ab324948'>ab324948</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HEK-293 transfected with siRNA specifically targeting eIF4G1 whole cell lysate at 20 µg

Lane 3:

L-929 (mouse connective tissue fibroblast) whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 5:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control fresh whole cell lysate at 20 µg

Lane 6:

HEK-293 transfected with siRNA specifically targeting eIF4G1 fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 260 kDa,124 kDa

false

• WB

Supplier Data

Western blot - Anti-eIF4G1 antibody [EPR30302-554] - BSA and Azide free (AB324959)

This data was developed using ab324948, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9819442; PMID : 36897548).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The bands beneath the target band (260 kDa) are likely to be degradation products.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-eIF4G1 antibody [EPR30302-554] (<a href='/products/primary-antibodies/eif4g1-antibody-epr30302-554-ab324948'>ab324948</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) fresh whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) fresh whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) fresh whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 260 kDa,124 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-eIF4G1 antibody [EPR30302-554] - BSA and Azide free (AB324959)

This data was developed using ab324948, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : kidney.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9819442; PMID : 36897548).

The bands beneath the target band (260 kDa) are likely to be degradation products.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-eIF4G1 antibody [EPR30302-554] (<a href='/products/primary-antibodies/eif4g1-antibody-epr30302-554-ab324948'>ab324948</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Lane 5:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 260 kDa,124 kDa

false

Exposure time: 15s